Catf6

Antibodies for KDEL receptor (sc-33806, 1:50 dilution for immunostaining), Bax (sc-493, 1:500 dilution for western blot), and Bcl2 (sc-7382, 1:1000 dilution for western blot) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for CHOP (#2895, 1:1000 dilution for western blot), cleaved caspase-3 (#9662, 1:1000 dilution for western blot), Ero1α (#3264, 1:1000 dilution for western blot), sXBP1 (#12782, 1:1000 dilution for western blot), and cleaved PARP (#9542, 1:1000 dilution for western blot) were from Cell Signaling Technology (Boston, MA, USA). Antibodies for BiP (ab21685, 1:500 dilution for western blot), cATF6 (ab11909, 1:1000 dilution for western blot), and GAPDH (ab9484, 1:1000 dilution for western blot) were from Abcam (Cambridge, MA, USA). Antibodies for AGGF1 (11889-1-AP, 1:1000 dilution for western blot), ATF4 (10835-1-AP, 1:500 dilution for western blot), β-actin (60008-1-Ig, 1:1000 dilution for western blot), DR5 (15497-1-AP, 1:500 dilution for western blot), and Puma (55120-1-AP, 1:500 dilution for western blot) were from Proteintech (Wuhan, Hubei, China). An antibody for p-PERK (DF7576, 1:1000 dilution for western blot) was from Affinity Biosciences (Cincinnati, OH, USA), and an antibody for p-eIF2α (1:200 dilution for western blot) was from Bioss (Beijing, China).

The concentration of the protein sample was examined using a BCA protein assay kit (Beyotime, Shanghai, China). Different molecules were separated by SDS/PAGE (10%) and were transferred into PVDF membranes (Bio-Rad, Hercules, CA, USA). Antibodies against cATF6 (Abcam, Cambridge, UK), CHOP, β-catenin, Bcl-xL, Bcl-2, Cleaved caspase-3, Bax, p-Akt, p-NF-κB p65, p-IKBα, p-IKKα/β, GAPDH, β-actin (Cell Signaling Technology, Danvers, MA, USA) were added to incubate membranes at 4 °C overnight. Secondary antibodies conjugated with HRP (Cell Signaling Technology) were then applied to react with the membrane at room temperature for 1 h. Finally, SuperSignal West Pico Chemiluminescent Substrates (Thermo Fisher Scientific, Rockford, IL, USA) were dropped onto the PVDF membrane to enhance the detection signal of the protein bands.