Pcsk9 elisa kit

Manufactured by R&D Systems

The PCSK9 ELISA kit is a laboratory tool designed to detect and quantify the PCSK9 protein in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to provide a quantitative measurement of PCSK9 levels.

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Lab products found in correlation

Microplate reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Human PCSK9 Quantikine ELISA Kit Human PCSK9 ELISA kit Mouse PCSK9 ELISA kit Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit PCSK9 Quantikine ELISA Kit PCSK9 ELISAs

2 protocols using pcsk9 elisa kit

Quantification of secreted PCSK9

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Secreted PCSK9 in culture medium of HepG2, primary mouse hepatocytes and in serum of C57BL/6J mice were measured using human or mouse PCSK9 ELISA kit obtained from R&D System according to the instruction.

Dong B., Singh A.B., Kelvin Kan C.F, & Liu J. (2014). CETP inhibitors downregulate hepatic LDL receptor and PCSK9 expression in vitro and in vivo through a SREBP2 dependent mechanism. Atherosclerosis, 235(2), 449-462.

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Quantification of PCSK9 Levels

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PCSK9 levels in cell culture medium and serum were measured with a human or mouse PCSK9 ELISA Kit (R&D Systems) according to the manufacturer’s protocols. Briefly, cell culture supernatants or serum samples were diluted and incubated in precoated plates for 2–4 hours. After 2 hours of incubation with horseradish peroxidase–conjugated secondary antibody, TMB substrate solution was added and samples were incubated further for 30 minutes before stop buffer was added. Plates were read immediately by using a microplate reader (BioTeK) set at 450 nM.

Dong J., He M., Li J., Pessentheiner A., Wang C., Zhang J., Sun Y., Wang W.T., Zhang Y., Liu J., Wang S.C., Huang P.H., Gordts P.L., Yuan Z.Y., Tsimikas S, & Shyy J.Y. (2020). microRNA-483 ameliorates hypercholesterolemia by inhibiting PCSK9 production. JCI Insight, 5(23), e143812.

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