Coolpix 7900

Several colonies of Pachyseris were collected at various localities in the Red Sea and in the Indo-Pacific. Digital images of living corals in situ were taken with a Canon G9 in an Ikelite underwater housing or a Nikon Coolpix 7900 in a Nikon WP-CP4 waterproof case. From each coral specimen collected, a 2 cm² fragment was preserved in either 95% ethanol or CHAOS solution (Sargent et al. 1986 (link), Fukami et al. 2004 (link)) for molecular analyses. After tagging, the remaining corallum was bleached in sodium hypochlorite for 48h to remove soft parts, rinsed in freshwater, and air-dried for morphological analyses. Images of the cleaned skeletons were taken with a Canon G9 digital camera. Samples were identified following Veron and Pichon (1980) , Scheer and Pillai (1983) , Veron (1986) , Sheppard and Sheppard (1991) , and Nishihira and Veron (1995) . Two colonies of Leptoseris foliosa from New Caledonia already studied by Benzoni et al. (2012) were also included in the following analyses.

A mouse tail model of lymphedema was created as described previously [19 (link)]. Briefly, mice were anesthetized by intraperitoneal (i.p.) injection of mixed anesthetic agents containing 4.0 mg/kg midazolam (Sandoz, a Novartis division, Basel, Switzerland), 0.75 mg/kg medetomidine hydrochloride (Nippon Zenyaku Kogyo, Fukushima, Japan), and 5.0 mg/kg butorphanol (Meiji Seika Pharma, Tokyo, Japan). To remove the dermal lymphatic vessels, a 5 mm-wide circumferential incision was made through the dermis 10 mm distal to the base. The effects of medetomidine were then reversed with an i.p. injection of 0.75 mg/kg atipamezole (Nippon Zenyaku Kogyo). The distal part of the incision was digitally photographed (Nikon COOLPIX 7900, Tokyo, Japan) weekly. The diameters of the maximum horizontal tails at the distal edge of the incision were measured. The rate of reduction in the tail diameter was calculated.