PV-9000 two-step immunohistochemical kit and DAB color reagent (Zsbio) were used to detect indicated antigens in mouse tissue samples or human NSCLC tissue microarrays (TMA) (US Biomax, LC121c) according to the manufacturers’ instruction. Expression levels of SNRPB and RAB26 were evaluated by pathologists using H-score system.
Pv 9000 two step immunohistochemical kit
Manufactured by ZSGB-BIO
The PV-9000 two-step immunohistochemical kit is a laboratory equipment used for the detection and visualization of target proteins or antigens in tissue samples. It utilizes a two-step process involving a primary antibody and a secondary antibody conjugated with a detection system, enabling the specific labeling and identification of the target analyte.
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2 protocols using pv 9000 two step immunohistochemical kit
1
Immunohistochemical Detection of SNRPB and RAB26
2
Immunohistochemical Analysis of β3GNT2 Expression
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Tissues were fixed in formalin and embedded in paraffin. Afterward, 5 µm sections were prepared for IHC staining. PV-9000 two-step immunohistochemical kit (ZSGB-BIO, Beijing China) was used to detect the expression of β3GNT2 protein. The positive signal was visualized by DAB detection Kit (ZSGB-BIO). Slides were read by two pathologists using a Nikon Eclipse E600 microscope (Nikon, Tokyo, Japan). IHC score = intensity score × percentage score [12 (link)]. β3GNT2 expression was defined as follows: low expression (score 0–4) and high expression (score ≥ 5).
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