Anti parg

Protein concentration was determined using Bradford Protein Assay Kit (BioRad, Hercules, CA, United States). Fifteen micrograms of protein samples were run on a resolving 10% acrylamide/polyacrylamide gel and transferred to a PVDF membrane. All primary antibodies (anti-PSMA diluted 1:1,000; anti-PRMT2 diluted 1:500; anti-PARP diluted 1:1,000; anti-PARG diluted 1:1,000; anti-CPT1alpha diluted 1:1,000; anti-PGC1alpha diluted 1:1,000; anti-βactin diluted 1:5,000) from Cell Signaling Technology (Danvers, MA, United States) were incubated at 4°C overnight. After washing, membranes were incubated with the secondary antibodies (1: 5,000 diluted horseradish peroxidase (HRP)-anti-mouse (Sigma-Aldrich (St. Louis, MO, United States) and 1:1,000 diluted anti-rabbit IgG (Cell Signaling Technology (Danvers, MA, United States). The bands were visualized by a chemiluminescence reagent (Cell Signaling Technology). Gel imaging Chemidoc MP System (BioRad, Hercules, CA, United States) was used to visualize and examine the protein bands. Bands were quantified using Scion Image 4.0 (Scion Corporation, Chicago, Illinois, United States).

The following antibodies were used: anti-PAR, mouse monoclonal, Trevigen, 4335-MC-100; anti-PAR (10H), mouse monoclonal, Enzo Life Sciences, ALX-804-220; anti-ubiquitin, mouse monoclonal, Santa Cruz, sc-8017; anti-His tag, mouse monoclonal, Abcam, ab18184; anti-His tag, rabbit monoclonal, Cell signaling, 12698; anti-TOP1, mouse monoclonal, BD Biosciences, 556597; anti-dsDNA, mouse monoclonal, Abcam, ab27156; anti-FLAG, mouse monoclonal, Sigma Aldrich, F1804; anti-FLAG, rabbit polyclonal, Sigma Aldrich, F7425; anti-TDP1, rabbit polyclonal, Bethyl Laboratories, A301-618A; anti-PARP1 (F2), mouse monoclonal, Santa Cruz, sc-8007; anti-PARG, rabbit monoclonal, Cell Signaling, 66564; anti-γH2AX, rabbit polyclonal, Cell Signaling, 66564; anti-BrdU, mouse monoclonal, Abcam, ab8152; anti-USP7, rabbit polyclonal, Bethyl Lab, A300-033A; Rabbit TrueBlot: anti-rabbit IgG HRP, Rockland, 18-8816-31; Mouse TrueBlot ULTRA: anti-mouse Ig HRP, Rockland, 18-8817-31; anti-Rabbit IgG, Alexa Fluor 568, Thermo Fisher, A-11011; anti-Mouse IgG, Alexa Fluor 488, Thermo Fisher, A-28175.