The Brunello knockout pooled library was a gift from David Root and John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide-puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW-EFSp-Cas9-P2A-Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). mitoBFP was a gift from Gia Voeltz (Addgene # 49151). pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid #111759). pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid #131626). pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150) Other vectors generated during this study are available upon request.
Phage mt mkeima
Manufactured by Addgene
The PHAGE-mt-mKeima is a plasmid that encodes a mitochondrial-targeted variant of the fluorescent protein mKeima. mKeima is a pH-sensitive fluorescent protein that can be used to monitor mitochondrial pH changes.
Automatically generated - may contain errors
Lab products found in correlation
Pcmv vsv g, by Addgene (2 mentions)
Lentiguide puro, by Addgene (2 mentions)
Pspax2, by Addgene (2 mentions)
Pfugw efsp cas9 p2a zeo pawp30, by Addgene (2 mentions)
Brunello knockout pooled library, by Addgene (2 mentions)
Mito bfp, by Addgene (2 mentions)
Pgw1 mcherry egfp pim, by Addgene (2 mentions)
Lenticrisprv2 puro, by Addgene (2 mentions)
Plko 1 hygro, by Addgene (2 mentions)
Plenti cmv gfp puro 658 5, by Addgene (1 mentions)
Plenti cmv gfp puro, by Addgene (1 mentions)
2 protocols using phage mt mkeima
1
Shared Plasmid Resource Protocols
2
Plasmids for CRISPR and Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Brunello knockout pooled library was a gift from David Rootand John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide-puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW-EFSp-Cas9-P2A-Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658–5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). mito-BFP was a gift from Gia Voeltz (Addgene # 49151). pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid #111759). pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid #131626). pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150) Other vectors generated during this study are available upon request.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!