This procedure was based on previous studies [9 (link), 81 , 82 (link)]. Rats were habituated to experimenter handling and the open field arena. Rats were fasted 16 h before the experiment. On the day of the experiment, rats received a continuous i.v. infusion of vehicle (buffered saline), (S)-ketamine (10 mg/kg), or HNK (10 mg/kg) over 40 min in an open field arena. Ten minutes after start of infusion, rats were injected (IP) with 13 MBq of 2-deoxy-2-[18F]-fluoro-D-glucose (FDG; Cardinal Health). After 30 min, rats were anesthetized with 1.5% isoflurane, placed on a custom-made bed of a nanoScan small animal PET/CT scanner (Mediso Medical Imaging Systems) and scanned for 20 min on a static acquisition protocol, followed by a CT scan. The PET data were reconstructed and coregistered to an MRI template using PMOD4.2 software environment (PMOD Technologies, Switzerland). The standardized uptake value ratio (SUVRWB) images were calculated using the whole brain as a reference region. All statistical parametric mapping analyses were performed using Matlab R2016 (Mathworks) and SPM12 (University College London). The ROI-based data was obtained using PMOD and the Schiffer rat VOI-atlas[83 (link)] and the statistical analyses were performed using GraphPad’s Prism 9.
Pmod4.2 software environment
Manufactured by PMOD Technologies
Sourced in Switzerland
PMOD4.2 is a software environment for the analysis of biomedical images. It provides a comprehensive set of tools for the visualization, quantification, and modeling of data acquired from various imaging modalities, including PET, SPECT, CT, and MRI.
Automatically generated - may contain errors
2 protocols using pmod4.2 software environment
1
PET Imaging of Ketamine and Metabolite Effects
2
PET Imaging of Ketamine Metabolism
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This procedure was based on previous studies [9, (link)81, 82] . Rats were habituated to experimenter handling and the open field arena. Rats were fasted 16 h before the experiment. On the day of the experiment, rats received a continuous i.v. infusion of vehicle (buffered saline), (S)-ketamine (10 mg/kg), or HNK (10 mg/kg) over 40 min in an open field arena. Ten minutes after start of infusion, rats were injected (IP) with 13 MBq of 2-deoxy-2-[18F]-fluoro-D-glucose (FDG; Cardinal Health). After 30 min, rats were anesthetized with 1.5% isoflurane, placed on a custom-made bed of a nanoScan small animal PET/CT scanner (Mediso Medical Imaging Systems) and scanned for 20 min on a static acquisition protocol, followed by a CT scan. The PET data were reconstructed and coregistered to an MRI template using PMOD4.2 software environment (PMOD Technologies, Switzerland). The standardized uptake value ratio (SUVR WB ) images were calculated using the whole brain as a reference region. All statistical parametric mapping analyses were performed using Matlab R2016 (Mathworks) and SPM12 (University College London). The ROI-based data was obtained using PMOD and the Schiffer rat VOIatlas [83] and the statistical analyses were performed using GraphPad's Prism 9.
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