Dar00

Manufactured by R&D Systems

The DAR00 is a laboratory instrument designed for cell culture applications. It features temperature and gas flow control capabilities to support optimal cell growth conditions.

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Lab products found in correlation

Deg00, by R&D Systems (1 mentions) Dtga00, by R&D Systems (1 mentions)

2 protocols using dar00

ELISA Analysis of Secreted Growth Factors

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HDMEC and tumor cells were cultured with serum free medium (EBM and DMEM, respectively) for 24 hours. The supernatant was collected and ELISA for EGF (Cat. DEG00, R&D Systems), TGF-alpha (Cat. DTGA00, R&D Systems) and Amphiregulin (Cat. DAR00, R&D Systems) was performed.

Zhang Z., Dong Z., Lauxen I.S., Filho M.S, & Nör J.E. (2014). Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype. Cancer research, 74(10), 2869-2881.

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Amphiregulin Secretion Quantification

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As above, cells were serum-starved for 18 h prior to 10 min of NE stimulation. Supernatants were collected, centrifuged at 2000 rpm for 10 min at 4°C to remove cellular debris, and frozen at -80°C in aliquots. Amphiregulin protein was measured using sandwich ELISA (DAR00, R&D Systems). The absorbance at 450 nm was read with a reference wavelength of 540 nm using an ELISA plate reader.

Xiao Z, & Hammes S.R. (2021). AXL cooperates with EGFR to mediate neutrophil elastase-induced migration of prostate cancer cells. iScience, 24(11), 103270.

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