Ras 27h5

Manufactured by Cell Signaling Technology

Ras(27H5) is a mouse monoclonal antibody that recognizes the Ras protein, a small GTPase that plays a critical role in cellular signaling pathways. The antibody is suitable for use in various immunological applications, including Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

2 protocols using ras 27h5

Tau Phosphorylation and Signaling Assays

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DHCR24 (#2033, Cell Signaling Technology), Tau5 (AHB0042, Thermo), Phospho-Tau (Thr181) (ab254409, Abcam), Phospho-Tau (Ser199) (#29,957, Cell Signaling Technology), Phospho-Tau (Ser396) (#9632, Cell Signaling Technology), Actin (#2118, Proteintech), cavin1 (#18,892–1-AP, Proteintech), Phospho-Tau (Ser262)(ab131354, Abcam), Phospho-Tau (Thr231)(#4137, ABclonal), Ras(27H5)(#3339, Cell Signaling Technology), p44/42 MAPK (ERK1/2) (137F5)(#4695S, Cell Signaling Technology), Phospho-p44/42 MAPK (ERK1/2)(Thr202/Tyr204)(#4730S, Cell Signaling Technology), MEK1/2 (D1A5)(#8727, Cell Signaling Technology), Phospho-MEK1/2 (Ser217/221)(#9154, Cell Signaling Technology), U0126 (#9903, Cell Signaling Technology), Propidium Iodide (abs9105, Absin), Methyl-β-cyclodextrin (abs42021762, Absin), Filipin III (abs42018484, Absin), Oil Red O (CAS:1320–06-5, O0625-25G, Sigma), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L)(SA00001-2, Proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L)(SA00001-1, Proteintech).

Mai M., Guo X., Huang Y., Zhang W., Xu Y., Zhang Y., Bai X., Wu J, & Zu H. (2022). DHCR24 Knockdown Induces Tau Hyperphosphorylation at Thr181, Ser199, Ser262, and Ser396 Sites via Activation of the Lipid Raft-Dependent Ras/MEK/ERK Signaling Pathway in C8D1A Astrocytes. Molecular Neurobiology, 59(9), 5856-5873.

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Growth Factor Signaling Pathway Analyses

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Recombinant human fibroblast growth factor 9 (FGF9; #273-F9-025), hepatocyte growth factor (HGF; 294-HG), transforming growth factor alpha (TGFα; 239-A) and epidermal growth factor (EGF; 236-EG) were purchased from R&D systems. Antibodies raised against phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (#9101), p44/42 MAPK (ERK1/2) (#9102), α-Tubulin (11H10) (#2125), phospho-EGF receptor (Tyr1068) (D7A5) (#3777), EGF receptor (D38B1) XP® rabbit mAb 1:1000 (#4267), GRB2 rabbit antibody (#3972), E-Cadherin (24E10) (#3195), β-Actin (13E5) (#4970), hnRNP A0 (#4046), RAS (27H5) (#3339), and GST (26H1) (#2624), (#7076) were purchased from Cell Signalling, as was an anti-rabbit IgG HRP-linked antibody (#7074). An anti-RFP rabbit antibody (ab62341) was purchased from Abcam and an anti-SOS1 mouse antibody (WH0006654M1) purchased from Sigma-Aldrich. DH5α competent and One Shot™ E. coli were purchased from Thermo Fisher Scientific. BL21 (DE3) competent E. Coli were purchased from NEB.

Seiler C., Stainthorp A.K., Ketchen S., Jones C.M., Marks K., Quirke P, & Ladbury J.E. (2022). The Grb2 splice variant, Grb3-3, is a negative regulator of RAS activation. Communications Biology, 5, 1029.

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