Tanespimycin was provided by LC Laboratories (Woburn, MA). Docetaxel was purchased from American Radiolabeled Chemicals, Inc. (St. Louis) and Rapamycin, TSA, 3-MA and Z-vad reagents were from Cell Signaling Technology (Danvers, MA). Each of these compounds was dissolved in dimethyl sulfoxide (DMSO) at a given concentration and aliquots were stored at − 20 °C. Stock solutions were diluted to the final appropriate concentrations just before use.
Mouse anti-caspase-3 monoclonal antibody was purchased from Imegenex (San Diego, CA). Mouse anti-caspase-8, rabbit anti-caspase-9, rabbit anti-LC3B-II and rabbit anti-poly (ADP-ribose) polymerase (PARP) monoclonal antibodies were purchased from Cell Signaling (Cell Signaling Technology, Danvers, MA). Mouse anti-Hsp90α and Rabbit anti-Hsp90β monoclonal antibodies were from Abcam (Cambridge, UK). Mouse anti-HDAC6, goat anti-TAT1 and mouse anti-β-actin polyclonal antibodies were from Sigma (Santa Cruz Biotechnology, Santa Cruz, CA). Mouse anti-Ac-α-tubulin and α-tubulin polyclonal antibodies were from Cell Signaling (Cell Signaling Technology, Danvers, MA).
Mouse anti-caspase-3 monoclonal antibody was purchased from Imegenex (San Diego, CA). Mouse anti-caspase-8, rabbit anti-caspase-9, rabbit anti-LC3B-II and rabbit anti-poly (ADP-ribose) polymerase (PARP) monoclonal antibodies were purchased from Cell Signaling (Cell Signaling Technology, Danvers, MA). Mouse anti-Hsp90α and Rabbit anti-Hsp90β monoclonal antibodies were from Abcam (Cambridge, UK). Mouse anti-HDAC6, goat anti-TAT1 and mouse anti-β-actin polyclonal antibodies were from Sigma (Santa Cruz Biotechnology, Santa Cruz, CA). Mouse anti-Ac-α-tubulin and α-tubulin polyclonal antibodies were from Cell Signaling (Cell Signaling Technology, Danvers, MA).