Rxi 5ht capillary column

GC-MS analysis was performed using an Agilent 7890 B gas chromatograph equipped with a 5977B mass selective detector (GC/MSD) (Agilent Technologies, Santa Clara, CA, USA). Chromatographic separation was achieved using a Rxi-5HT capillary column (30 m × 0.25 mm I.D., 0.25 μm film thickness; RESTEK, Bellefonte, PA, USA). An autosampler (Agilent Technologies; 7683B) was used for all experiments. The temperature of the injector was 280 °C. Using split mode, 1 µL of each extract was injected at a ratio of 1/50. The carrier gas was helium C-60 at a constant flow rate of 1 mL/min. The GC oven temperature was initially 60 °C for 1 min, increased to 160 °C in 3 °C/min increments, and then increased to 320 °C in 10 °C/min increments, followed by a hold for 10 min. The mass spectrometer used the electron ionization mode at 70 eV with the ion source temperature set at 250 °C. The running time was 30 min. Scan mode was used in the range of 30–600 m/z with a scan rate of 2.6 scan/s. Agilent Mass Hunter Qualitative Analysis B.04.00 software was used for data analysis. Single compounds were identified by comparing mass spectra with NIST mass spectral libraries (National Institute of Standards, 2020 version) and the Wiley Registry 12th Edition.

GC–MS analysis was performed using an Agilent 7890 B gas chromatograph equipped with a 5977B mass selective detector (GC/MSD) (Agilent Technologies, Santa Clara, CA, USA). Chromatographic separation was achieved using a Rxi-5HT capillary column (30 m × 0.25 mm I.D., 0.25 μm film thickness; RESTEK, Bellefonte, PA, USA). An automatic autosampler (Agilent 7683B) was used for all experiments. The temperature of the injector was 280 °C. One microliter of each extract was injected in split mode with a ratio of 1/50. The carrier gas was helium C-60 at a constant flow of 1 mL/min. The GC oven temperature was initially 60 °C for 1 min, increased to 160 °C in 3 °C/min increments, and then increased to 320 °C in 10 °C/min increments, followed by a hold for 10 min. The mass spectrometer was tuned on electron ionization mode at 70 eV with the ion source temperature set at 250 °C. The running time was 30 min. Scan mode was used in the range of 30–600 m/z with a scan rate of 2.6 scan/s. The Agilent Mass Hunter Qualitative Analysis B.04.00 software was used for data analysis. Single compounds were identified by comparing mass spectra with NIST mass spectral libraries (National Institute of Standards, 2005 version) and the Wiley Registry 8th Edition.