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Sc 22536

Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States

The Sc-22536 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed for specific laboratory applications, but a detailed and unbiased description is not available at this time.

Automatically generated - may contain errors

2 protocols using sc 22536

1

Immunoblotting of Bone Markers

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Immunoblotting was performed as previously described [16 (link)]. Equal amounts of tissue lysates were used for immunoblotting. Blots were incubated with specific antibodies to Foxc2, CD31 (Abcam, ab5060, ab182982. Cambridge, UK), Osterix (Santa Cruz Biotechnology, sc-22536), and Osteocalcin (ThermoFisher Scientific, PA1-511A). β-actin (Sigma-Aldrich, A2228) was used as a loading control.
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2

Immunoblotting of Bone and Endothelial Markers

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Immunoblotting was performed as previously described [20 (link)]. Equal amounts of tissue lysates were used for immunoblotting. Blots were incubated with specific antibodies to Foxc2, CD31, and aurora (Abcam, ab5060, ab182982 and ab13824, Cambridge, UK), Osterix (Santa Cruz Biotechnology (Dallas, TX, USA), sc-22536), Osteocalcin, and glucocorticoid receptor (ThermoFisher Scientific, PA1-511A and MA1-510). β-actin (Sigma-Aldrich, A2228) was used as a loading control.
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