The overnight culture of S. pyogenes, grown on MH agar at 37°C, was added to a saline solution, containing 0.5% Tween-80 (Sigma-Aldrich). Different concentrations of the A. vestita essential oil (5, 25 and 50 μg/ml) were prepared and added to the suspension, which was incubated at room temperature. After 12 h, the bacterial cells were centrifuged at 15,000 × g for 20 min. The bacterial cells were washed with 0.5 mol/l Tris-acetate buffer (pH 7.1; Sigma-Aldrich), fixed in Tris-acetate buffer containing 1.2% glutaraldehyde (Sigma-Aldrich), and were then freeze-dried. The bacterial culture of S. pyogenes was then visualized using SEM (Hitachi S-3000H; Hitachi, Ltd., Tokyo, Japan) at magnification, ×10,000. The bacterial cell suspension in saline with no essential oil treatment served as a negative control.
Tris acetate buffer
Manufactured by Merck Group
Tris-acetate buffer is a common laboratory buffer used to maintain a stable pH environment in various biochemical and molecular biology applications. It is composed of tris(hydroxymethyl)aminomethane (Tris) and acetic acid, which together provide a buffering capacity in the physiologically relevant pH range. The specific formulation and concentration of the Tris-acetate buffer can be adjusted to suit the requirements of the experiment or application.
Automatically generated - may contain errors
Lab products found in correlation
Glutaraldehyde, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Phosphoric acid, by Thermo Fisher Scientific (1 mentions)
Hydroxylamine, by Merck Group (1 mentions)
Ethanol, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Citric acid, by Thermo Fisher Scientific (1 mentions)
L glutamic acid γ monohydroxamate, by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Cacl2, by Merck Group (1 mentions)
2 protocols using tris acetate buffer
1
Antimicrobial Effects of Artemisia Essential Oil
2
Soy Protein Isolate and Transglutaminase Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Soy
protein isolate (PROFAM 974) with 90%
protein, on a dry basis, was supplied by Archer Daniels Midland company
Protein Specialties Division, Netherlands. SPI had 5% moisture, 4%
fat, and 5% ash. Transglutaminase (Activa RM) was donated by Ajinomoto,
Japan. According to the data sheet provided by the company, Activa
RM contains 99.5% sodium caseinate and maltodextrin and 0.5% enzyme.
On the basis of our preliminary study, measured activity of the enzyme
was 47.8 unit per gram of enzyme.24 (link) Glycerol
and alkali lignin (code 370959) were purchased from Sigma-Aldrich
Inc. (St. Louis, MO). Calcium lignosulfonate was donated from Green
Agrochem, grade one, Jinan, China. Reagents for enzyme activity measurements
such as Tris–HCl, Tris-acetate buffer, CaCl2, glutathione,
hydroxylamine, CBZ-glutaminylglycine,l -glutamic acid γ-monohydroxamate,
and FeCl3-trichloroacetic were purchased from Sigma-Aldrich.
Citric acid, ethanol, methanol, BHT, and phosphoric acid were obtained
from Fisher Scientific. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) was obtained
from Sigma Chemical Co.
protein isolate (PROFAM 974) with 90%
protein, on a dry basis, was supplied by Archer Daniels Midland company
Protein Specialties Division, Netherlands. SPI had 5% moisture, 4%
fat, and 5% ash. Transglutaminase (Activa RM) was donated by Ajinomoto,
Japan. According to the data sheet provided by the company, Activa
RM contains 99.5% sodium caseinate and maltodextrin and 0.5% enzyme.
On the basis of our preliminary study, measured activity of the enzyme
was 47.8 unit per gram of enzyme.24 (link) Glycerol
and alkali lignin (code 370959) were purchased from Sigma-Aldrich
Inc. (St. Louis, MO). Calcium lignosulfonate was donated from Green
Agrochem, grade one, Jinan, China. Reagents for enzyme activity measurements
such as Tris–HCl, Tris-acetate buffer, CaCl2, glutathione,
hydroxylamine, CBZ-glutaminylglycine,
and FeCl3-trichloroacetic were purchased from Sigma-Aldrich.
Citric acid, ethanol, methanol, BHT, and phosphoric acid were obtained
from Fisher Scientific. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) was obtained
from Sigma Chemical Co.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!