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Rabbit anti zscan1

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Rabbit anti-ZSCAN1 is a polyclonal antibody raised in rabbits against the ZSCAN1 protein. ZSCAN1 is a zinc finger and SCAN domain-containing protein involved in cellular processes. The antibody can be used to detect and study the ZSCAN1 protein in various applications.

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Lab products found in correlation

Control rabbit igg, by Thermo Fisher Scientific (2 mentions) Rabbit anti zscan1, by Thermo Fisher Scientific (2 mentions)

2 protocols using rabbit anti zscan1

1

Profiling Candidate Autoantigens in ROHHAD

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To examine candidate autoantigen expression in ROHHAD tumor and healthy human brain, we used immunohistochemistry and immunoprecipitation‐mass spectrometry (IP‐MS), respectively. For tumor examination, fresh frozen tumor was embedded, sectioned, fixed on slides, stained, and imaged according to standard procedures. To detect ZSCAN1, we immunostained with commercial antibody to ZSCAN1 (rabbit, Thermo Fisher Scientific, PA552488) and anti‐rabbit secondary. For IP‐MS experiments, ~100 mg of frozen human hypothalamus was homogenized and prepared for IP and subsequent MS analysis. For IP, lysate was diluted to 0.5 mg/mL and 1 of the following antibodies was added at 1 μg/mL: rabbit anti‐ZSCAN1 (Thermo Fisher Scientific, PA552488), rabbit anti‐ZSCAN1 (Sigma Aldrich HPA007938), rabbit IgG control (Thermo Fisher Scientific, 31235). Positive identification of ZSCAN1 required detection with 2 commercial antibodies and absence from isotype control. Two technical replicates were performed.
Mandel‐Brehm C., Benson L.A., Tran B., Kung A.F., Mann S.A., Vazquez S.E., Retallack H., Sample H.A., Zorn K.C., Khan L.M., Kerr L.M., McAlpine P.L., Zhang L., McCarthy F., Elias J.E., Katwa U., Astley C.M., Tomko S., Dalmau J., Seeley W.W., Pleasure S.J., Wilson M.R., Gorman M.P, & DeRisi J.L. (2022). ZSCAN1 Autoantibodies Are Associated with Pediatric Paraneoplastic ROHHAD. Annals of Neurology, 92(2), 279-291.
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2

ZSCAN1 Expression in ROHHAD Tumor

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To examine candidate autoantigen expression in ROHHAD tumor and healthy human brain we used immunohistochemistry and immunoprecipitation-mass spectrometry (IP-MS), respectively. For tumor examination, fresh frozen tumor was embedded, sectioned, fixed on slides, stained and imaged according to standard procedures. To detect ZSCAN1 we immunostained with commercial antibody to ZSCAN1 (Rabbit, Thermo Fisher Scientific, PA552488) and anti-rabbit secondary. For IP-MS experiments, ~100 milligrams of frozen human hypothalamus was homogenized and prepared for IP and subsequent MS analysis. For IP, lysate was diluted to 0.5 mg/mL and one of the following antibodies was added at 1ug/mL: Rabbit anti-ZSCAN1 (Thermo Fisher Scientific, PA552488), Rabbit anti-ZSCAN1 (Sigma Aldrich HPA007938), Rabbit IgG control (Thermo Fisher Scientific, 31235). Positive identification of ZSCAN1 required detection with two commercial antibodies and absence from isotype control. Two technical replicates were performed.
Mandel‐Brehm C., Benson L.A., Tran B., Kung A.F., Mann S.A., Vazquez S.E., Retallack H., Sample H.A., Zorn K.C., Khan L.M., Kerr L.M., McAlpine P.L., Zhang L., McCarthy F., Elias J.E., Katwa U., Astley C.M., Tomko S., Dalmau J., Seeley W.W., Pleasure S.J., Wilson M.R., Gorman M.P, & DeRisi J.L. (2022). ZSCAN1 Autoantibodies Are Associated with Pediatric Paraneoplastic ROHHAD. Annals of Neurology, 92(2), 279-291.
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