Bicinchoninic acid bca protein assay kit p0012

NaF was purchased from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). Reagent kits for determination of biochemical parameters were purchased from Nanjing Jiancheng Bioengineering Institute of China (Nanjing, China). RNAiso Plus, Prim-Script^TM RT reagent Kit and SYBR^® Premix Ex Taq^TM II were obtained from Takara Biotechnology (Dalian) Co., Ltd. (Dalian, Liaoning, China). Radio-immunoprecipitation Assay (RIPA) lysis buffer (P0013C) and bicinchoninic acid (BCA) Protein Assay Kit (P0012) were purchased from Beyotime Biotechnology, China. RPMI 1640 (11875119) was supplied by Gibco, UK. The mouse NF-κB (ab32536), IκB (ab32518) and COX-2 (ab179800) were obtained from Abcam, UK. IL-6 (12912), p-NF-κB (3033), and anti-rabbit IgG (7074P2) were purchased from Cell Signaling Technology, USA. TNF-α (sc-1351) and IL-10 (sc-1783) were supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). IL-1β (abs115412), IL-4 (abs116760) and bicinchoninic acid (BCA) were obtained from Absin Bioscience Inc. (Absin, Shanghai, China). Anti-goat IgG antibody (BL004A) was supplied by Biosharp Bioscience Inc. (Biosharp, Anhui, China). Other chemicals including 75% ethanol, 100% ethanol, isopropyl alcohol and chloroform used in the experiment were of analytical grade.

Cadmium chloride (CdCl₂, 202908), NAC (30498), and DFO (D9533) were obtained from Sigma-Aldrich (Sigma, St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 12800-017), fetal bovine serum (FBS, Gibco, 10437-028), Trizol (Ambion, 15596026), and trypsin-EDTA (25200072) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The ROS detection kit (S0033S), Mito-Tracker Red fluorescent probe (C1035), and Bicinchoninic acid (BCA) protein assay kit (P0012) were from Beyotime Biotechnology Co., Ltd. (Beyotime, Shanghai, China). The MDA (A003-1-2) and GSH detection kits (A006-2-1) were purchased from Nanjing Jiancheng Bio-Engineering Institute Co., Ltd. (Nanjing, China). The Cell Counting Kit-8 (CCK-8, A311-01) was purchased from Vazyme Biotechnology Co., Ltd. (Vazyme, Nanjing, China). The PrimeScrip^TM RT Reagent Kit (RR037A) and SYBR Green™ Premix Ex Taq™ (RR390A) were obtained from Takara Biomedical Technology Co., Ltd. (Takara, Beijing, China). The RIPA lysate (20101ES60) was obtained from New Cell & Molecule Biotechnology Co., Ltd. (NCM, Suzhou, China). The Fer-1 (HY-100579) and Erastin (HY-15763) were purchased from MedChemExpress LLC (MCE, Princeton, NJ, USA). All chemicals were of the highest purity grade available.