Pe rat anti mouse cd73

All cell culture media, trypsin, fetal bovine serum (FBS), and antibiotic/antimycotic solutions were obtained from Invitrogen (Carlsbad, CA, USA). The L-929 fibroblast (CCl-1), LADMAC macrophage/monocyte (CRL-2420), and C3H/HeJ mouse I-13.35 splenic cell lines (CRL-2471) deficient for TLR-4 were purchased from the American Type Tissue Collection (ATCC) (Manassas, VA, USA). Endotoxin tested (less than 0.1 ng/μg) IFNγ (#I1000) was purchased from US Biological (Salem, MA, USA), the inducible nitric oxide synthase (iNOS) (#2977) antibody from Cell Signaling Technology (Danvers, MA, USA), the anti-monocyte/macrophage antibody (MOMA-2, ab33451) and anti-integrin beta-1 antibody (CD29) (#ab23834) from Abcam (Cambridge, MA, USA), and the fluorescein isothiocyanate (FITC) rat anti-mouse CD44 (#553133), phycoerythrin (PE) rat anti-mouse CD105 (#562759), PE rat anti-mouse Ly-6AE (Sca-1) (#561076), FITC rat anti-mouse CD45 (#553080), FITC rat anti-mouse CD106 (#553332), PE rat anti-mouse CD73 (#557041), and FITC rat anti-mouse CD11b (#553310) were purchased from BD Biosciences (San Jose, CA, USA). Human ox-LDL was purchased from Intracel (Frederick, MD, USA). Gamma-irradiated LPS from Escherichia coli (#L4391) and all other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified.

The expression of CD73, CD14, CD34, and CD45 were evaluated on cells obtained from F-SMSCs or M-SMSCs. Cells (1×10⁶) were suspended in 2% BSA/PBS and labeled with PE rat anti-mouse CD73, PE rat anti-mouse CD14, FITC rat anti-mouse CD34, and FITC rat anti-mouse CD45 (all from BD, U.S.). Flow cytometry was performed using a FC500 flow cytometer (Beckman Coulter) and analyzed by Beckman Coulter CXP software.