Cpi 203

BET762, I-BET726, and CPI-203 were purchased from MedChemExpress (NJ, USA), cell counting Kit-8 (CCK-8) was purchased from Bimake (Houston, TX, USA), and Transwell Chambers were purchased from Corning (Corning, NY, USA). Anti-histone crotonylation (Kcr) antibody, anti-histone acetylation modification (Kac) antibody, and anti-Histone H3 Lys18 crotonylation (H3K18cr) antibody at the 18th amino acid position of histone H3 were purchased from PTM-Biolabs (Hangzhou, China). Anti-actin (β-actin) antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA), anti-histone H3 antibody and BRD4 antibody were purchased from Abcam (Cambridge, UK), and anti-histone acetylase P300 antibody was purchased from AbSci (Vancouver, WA, USA). Anti-histone acetylase GCN5 antibody, anti-Cyclin D1 antibody, anti-HDAC1 antibody, anti-HDAC2 antibody, anti-HDAC3 antibody, anti-ACSS2 antibody, anti-AR antibody, and anti-PSA antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and C-MYC was purchased from Proteintech.

Human liver cancer cell lines HepG2, HepG2.2.15, SMMC‐7721, Huh‐7, and BEL7402; murine liver cancer cell line H22; and human breast cancer cell line MDA‐MB‐231 were obtained from the ATCC. The cells were cultured in DMEM/RPMI‐1640 medium, with 10% fetal bovine serum and 1% penicillin/streptomycin in culture conditions of 5% CO₂ in air at 37°C. CPI‐203 was purchased from MedChemExpress (MCE). Human antibodies specific for BRD4, GAPDH, and β‐actin were purchased from Cell Signaling Technology. Human anti‐PD‐L1 antibody was purchased from Abcam. Mouse antibody specific for PD‐L1 as well as recombinant human IFN‐γ were purchased from Proteintech. Mouse anti‐CD3–FITC, anti‐CD4–APC, anti‐CD8–PerCP‐Cy5.5, and anti‐PD‐L1–PE conjugated antibodies were purchased from BD Biosciences. PD‐1 antibody (αPD‐1, clone: RMP1‐14) and IgG2a isotype control (clone: 2A3) were purchased from BioXcell.