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Multiquant v1

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MultiQuant v1.1 is a software application developed by AB Sciex for the analysis of mass spectrometry data. The core function of MultiQuant is to provide a comprehensive platform for the quantification of target analytes in complex biological samples.

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Lab products found in correlation

Prominence uflc hplc system, by Shimadzu (2 mentions) 5500 qtrap hybrid triple quadrupole linear ion trap mass spectrometer, by AB Sciex (2 mentions)

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MultiQuant (84 citations)

2 protocols using multiquant v1

1

Targeted LC-MS/MS Metabolite Analysis

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Metabolite levels were determined by targeted liquid-chromatography tandem mass spectrometry (LC-MS/MS) analysis as described previously (Yuan et al., 2012 (link)). Briefly, 48 hr prior to each experiment, 2.5x105 HCT116 cells expressing shGFP or shPARK2 were seeded in 6-cm dishes. The media for each plate was replaced 2 hr prior to metabolite extraction and after aspiration, 4 mL pre-chilled (at −80°C) methanol was added to the cells on dry ice for 15 min. Cell extracts were collected into 15 mL conical tubes and centrifuged for 5 min at 4200 rpm. Solvent in the resulting supernatant was evaporated using a centrifugal vacuum evaporator (“SpeedVac”) and samples were re-suspended in 20 μL HPLC-grade water for mass spectrometry. 8 μL were injected and analyzed using a Prominence UFLC HPLC system (Shimadzu) for hydrophilic interaction liquid chromatography (HILIC), coupled to a QTRAP 5500 hybrid triple quadrupole/linear ion trap mass spectrometer (SCIEX) operated in the selected reaction monitoring (SRM) mode. Peak areas of LC-SRM-MS traces for each metabolite were integrated using the MultiQuant v1.1 software (SCIEX).
Gupta A., Anjomani-Virmouni S., Koundouros N., Dimitriadi M., Choo-Wing R., Valle A., Zheng Y., Chiu Y.H., Agnihotri S., Zadeh G., Asara J.M., Anastasiou D., Arends M.J., Cantley L.C, & Poulogiannis G. (2017). PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Molecular Cell, 65(6), 999-1013.e7.
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2

Quantitative Metabolite Profiling in HCT116 Cells

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Metabolite levels were determined by targeted liquid-chromatography tandem mass spectrometry (LC-MS/MS) analysis as described previously (Yuan et al., 2012 (link)). Briefly, 48 hours prior to each experiment, 2.5×105 HCT116 cells expressing shGFP or shPARK2 were seeded in 6-cm dishes. The media for each plate was replaced 2 hours prior to metabolite extraction and after aspiration, 4 mL pre-chilled (at −80 °C) methanol was added to the cells on dry ice for 15 min. Cell extracts were collected into 15 mL conical tubes and centrifuged for 5 min at 4200 rpm. Solvent in the resulting supernatant was evaporated using a centrifugal vacuum evaporator (“SpeedVac”) and samples were re-suspended in 20 μL HPLC-grade water for mass spectrometry. 8 μL were injected and analyzed using a Prominence UFLC HPLC system (Shimadzu) for hydrophilic interaction liquid chromatography (HILIC), coupled to a QTRAP 5500 hybrid triple quadrupole/linear ion trap mass spectrometer (SCIEX) operated in the selected reaction monitoring (SRM) mode. Peak areas of LC-SRM-MS traces for each metabolite were integrated using the MultiQuant v1.1 software (SCIEX).
Gupta A., Anjomani-Virmouni S., Koundouros N., Dimitriadi M., Choo-Wing R., Valle A., Zheng Y., Chiu Y.H., Agnihotri S., Zadeh G., Asara J.M., Anastasiou D., Arends M.J., Cantley L.C, & Poulogiannis G. (2017). PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Molecular Cell, 65(6), 999-1013.e7.
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