Varioskan lux microtiter plate reader
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Varioskan Lux is a microtiter plate reader designed for versatile and sensitive absorbance, fluorescence, and luminescence measurements. It features a xenon flash lamp light source and automated optics to provide fast and accurate read times across a wide range of applications.
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2 protocols using varioskan lux microtiter plate reader
1
Thiamine-Dependent GFP Expression
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L. lactis strains MG1363 and EPH01 were grown and prepared as described above. For fluorescence intensity measurements, the cells were diluted 1:20 in G-CDMPC. When testing the effect of various concentrations of thiamine, this medium was supplemented with different amounts of thiamine. The growth (increase in the OD600) and fluorescence signal (excitation at 485 nm and emission at 535 nm) were recorded every 10 min in 0.2-mL cultures in 96-well microplates incubated for 22 h at 30°C using a Varioskan Lux microtiter plate reader (Thermo Fisher Scientific Inc., MA, USA). Fluorescence signals were corrected for the background value of the growth medium. sfGFP signals, in relative fluorescence units (RFU), were normalized by the corresponding OD600 measurements to obtain RFU/OD600 values.
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2
Characterization of 3D Printed Dental Materials
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In addition to cell compatibility, both autoclaved and nonautoclaved Dental SG and High Temp materials were characterized for their autofluorescence and surface topography. Autofluorescence was determined from six locations across the plates at three excitation and emission wavelengths (355/455 nm, 488/555 nm, and 530/575 nm) corresponding to the commonly used fluorophores also employed in this study (Hoechst 33342, calcein AM, and PI), by using Varioskan LUX microtiter plate reader (Thermo Fisher Scientific, Waltham, MA). The topography of 3D printed surfaces was analyzed by FEI Quanta FEG scanning electron microscope (FEI, Hillsboro, OR) and by Dektak/XT profilometer (Bruker, Billerica, MA) using a 2 μm stylus tip and 10 mg stylus weight over 3 mm path in 60 s. In addition, the wettability differences between autoclaved and non-autoclaved Dental SG were determined based on water contact angle (WCA) measurement using a CAM200 Optical Contact Angle Meter (KSV instruments Ltd, Helsinki, Finland). In this case, Dental SG was spin coated (3000 rpm, 30 s) on a 3D printed Dental SG sheet and cured under UV-LED (450 nm, 60 s) to be able to create a smooth surface for WCA determination.
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