Pcdna3.1 slc7a11 expression plasmid

Manufactured by Genomed

Sourced in China

The PcDNA3.1-SLC7A11 expression plasmid is a laboratory tool used for the expression of the SLC7A11 gene. It contains the necessary genetic elements for the production of the SLC7A11 protein in cells.

Automatically generated - may contain errors

Lab products found in correlation

Si ythdf1, by GenePharma (2 mentions) Simettl3 1, by GenePharma (2 mentions) Simettl3 2, by GenePharma (2 mentions) Pex 3 mettl3 expression plasmid, by GenePharma (2 mentions) Sh mettl3, by GenePharma (2 mentions)

2 protocols using pcdna3.1 slc7a11 expression plasmid

Targeted Knockdown and Overexpression of METTL3

Check if the same lab product or an alternative is used in the 5 most similar protocols

The siRNAs for METTL3 and YTHDF1, and lentivirus for METTL3 knockdown were synthesized by GenePharma (Shanghai, China). The sequences were as follows: siMETTL3#1 (sense: 5′-GCUACCUGGACGUCAGUAUTT-3′, antisense: 5′-AUACUGACGUCCAGGUAGCTT-3′); siMETTL3#2 (sense: 5′-GGUUGGUGUCAAAGGAAAUTT-3′, antisense: 5′-AUUUCCUUUGACACCAACCTT-3′); siYTHDF1 (sense: 5′-GGAGAAUAACGACAACAAATT-3′, antisense: 5′-UUUGUUGUCGUUAUUCUCCTT-3′); and shMETTL3 (5′-GCAAGAATTCTGTGACTATGG-3′).
The pEX-3-METTL3 expression plasmid was synthesized by GenePharma (Shanghai, China). The pcDNA3.1-SLC7A11 expression plasmid was synthesized by Genomeditech (Shanghai, China).

Xu Y., Lv D., Yan C., Su H., Zhang X., Shi Y, & Ying K. (2022). METTL3 promotes lung adenocarcinoma tumor growth and inhibits ferroptosis by stabilizing SLC7A11 m6A modification. Cancer Cell International, 22, 11.

+ Open protocol

+ Expand

METTL3 and YTHDF1 RNA Silencing Constructs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The siRNAs for METTL3 and YTHDF1, and lentivirus for METTL3 knockdown were synthesized by GenePharma (Shanghai, China). The sequences were as follows:
siMETTL3#1 (sense: 5′-GCUACCUGGACGUCAGUAUTT-3′, antisense: 5′-AUACUGACGUCCAGGUAGCTT-3′); siMETTL3#2 (sense: 5′-GGUUGGUGUCAAAGGAAAUTT-3′, antisense: 5′-AUUUCCUUUGACACCAACCTT-3′); siYTHDF1 (sense: 5′-GGAGAAUAACGACAACAAATT-3′, antisense: 5′-UUUGUUGUCGUUAUUCUCCTT-3′); and shMETTL3 (5'-GCAAGAATTCTGTGACTATGG-3').
The pEX-3-METTL3 expression plasmid was synthesized by GenePharma (Shanghai, China). The pcDNA3.1-SLC7A11 expression plasmid was synthesized by Genomeditech (Shanghai, China).

Xu Y., Lv D., Yan C., Su H., Zhang X., Shi Y., & Ying K. (2021). METTL3 Promotes Lung Adenocarcinoma Tumorigenesis and Inhibits Ferroptosis by Stabilizing SLC7A11 m6A Modification.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!