All reactions were carried out in oven-dried glassware under an argon atmosphere. Flash column chromatography was performed with silica gel Merck 60 (230–400 mesh ASTM, Darmstadt, Germany). TLC analysis was performed on 0.25 mm Silicagel Merck 60 F254 plates. Melting points were determined on a hot stage microscope apparatus (AS ONE, ATM-01, Osaka, Japan and were uncorrected. NMR spectra were recorded on a JEOL DELTA 300 (Tokyo, Japan) or JEOLRESONANCE ECX-500 spectrometer (Tokyo, Japan), operating at 300 MHz or 500 MHz for 1H-NMR and 75 MHz 120 MHz for 13C-NMR. Chemical shifts (δ ppm) in CDCl3 were reported downfield from TMS (= 0) for 1H-NMR. For 13C-NMR, chemical shifts were reported in the scale relative to CDCl3 (77.00 ppm) as an internal reference.IR Spectra were recorded on a JASCO FT/IR-5300 spectrophotometer (Tokyo, Japan). Mass spectra were measured on a JEOL JMS-T100LC spectrometer (Tokyo, Japan). HPLC data were obtained on a SHIMADZU HPLC system (consisting of the following: LC-20AT, CMB20A, CTO-20AC, and detector SPD-20A measured at 254 nm, Kyoto, Japan) using Daicel Chiracel AD-H or Ad-3 column (25 cm) at 25 °C. Optical rotations were measured on a JASCO DIP-370 (Na lamp, 589 nm, Tokyo, Japan).
Jms t100lc spectrometer
Manufactured by JEOL
Sourced in Japan
The JMS-T100LC spectrometer is a compact mass spectrometer designed for routine laboratory analysis. It features a high-performance ion source and a sensitive detector to provide accurate mass measurements. The instrument is capable of analyzing a wide range of sample types and can be used for applications such as chemical analysis, environmental monitoring, and materials characterization.
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Lab products found in correlation
Ft ir 5300 spectrophotometer, by Jasco (2 mentions)
Atm 01, by As One (1 mentions)
Hplc system, by Shimadzu (1 mentions)
Ecx 500 spectrometer, by JEOL (1 mentions)
Stearic acid, by Fujifilm (1 mentions)
4 dimethylaminopyridine, by Fujifilm (1 mentions)
Av 500 spectrometer, by Bruker (1 mentions)
P 2200 polarimeter, by Jasco (1 mentions)
Jnm al300, by JEOL (1 mentions)
Jnm eca500, by JEOL (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Jnm ecx400, by JEOL (1 mentions)
Jms sx102a, by JEOL (1 mentions)
Amx 500 spectrometer, by Agilent Technologies (1 mentions)
Dip 370 polarimeter, by Jasco (1 mentions)
5 protocols using jms t100lc spectrometer
1
Synthesis and Characterization of Organic Compounds
2
Synthesis of Click-Functionalized Amphiphiles
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11-Azido-3,6,9-trioxaundecan-1-amine (>93.0%), 10-undecynoic acid (>98.0%), tetraethylene glycol monomethyl ether (>98.0%), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (>98.0%) were purchased from TCI (Japan). Stearic acid (>95.0%), 4-dimethylaminopyridine (>99.0%), and CuCl (>95.0%) were procured from Wako Chemicals (Japan). N,N'-dicyclohexylcarbodiimide (>98.0%) was purchased from Kanto Chemical (Japan). All solvents and reagents were used as received. 1H- and 13C-NMR spectra were recorded on an AV-500 spectrometer (Bruker, USA) operating at 500 MHz and 125 MHz respectively. CDCl3 was used as the solvent. 1H-NMR spectra were referenced to tetramethylsilane (TMS) and 13C-NMR spectra were referenced to the deuterated solvent CDCl3. High resolution electrospray ionization mass spectrometry (HR-ESI-MS) was performed by using a JMS-T100LC spectrometer (JEOL, Japan).
3
Flash Chromatography and Spectroscopic Analysis
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Flash chromatography was performed on Silica gel 60 (spherical, particle size 0.040–0.100 mm; Kanto Co., Inc., Tokyo, Japan). Preparative-TLC (PLC) was performed using PLC Silica gel 60 F254 (0.5 mm, Merck Ltd., Darmstadt, Germany). Optical rotations were measured on a JASCO P-2200 polarimeter. 1H- and 13C-NMR spectra were recorded on JEOL JNM-AL300 (300 MHz), JEOL JNM-ECX 400 (400 MHz), and JEOL JNM-ECA 500 (500 MHz). The spectra were referenced internally according to the residual solvent signals of CDCl3 (1H-NMR, δ = 7.26 ppm; 13C-NMR, δ = 77.0 ppm) and DMSO-d6 (1H-NMR, δ = 2.50 ppm; 13C-NMR, δ = 39.5 ppm). Dates for 1H-NMR were recorded as follows: Chemical shift (δ, ppm), multiplicity (s, singlet; t, triplet; m, multiplet; br, broad), integration, and coupling constant (Hz). Dates for 13C-NMR were reported in terms of chemical shift (δ, ppm). Mass spectra were recorded on a JEOL JMS-T100LC spectrometer with ESI-MS mode, using MeOH as solvent.
4
Characterization of Novel Compounds
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All reagents used were purchased commercially from Aldrich Chemical. Co. Inc. (St Louis, MO, USA), and used without further purification. Solvents were supplied by J.T. Baker (Phillipsburg, NJ, USA), which were dried and distilled prior to use, using standard procedures established under dinitrogen atmosphere. The melting points were determined (without corrections) using a MELT-TEMP II Laboratory Devices, vibrational spectroscopy IR were performed in the range of υ 4000 to 350 cm−1 in a NICOLETMAGNA spectrometer 750 FT-IR in KBr tablet. EI-MS and DART ± MS were carried out using a JEOL JMS-SX102A and JEOL JMS-T100LC spectrometer, respectively. NMR spectra were recorded in CDCl3 at room temperature on a JEOL spectrometer GX300 ECLIPSE at 300 MHz frequency for 1H and 75 MHz for 13C{1H}. The chemical shifts (δ) for 1H and 13C are reported in ppm down field of Si (Me)4 (δ = 0.0). The abbreviations used in the description of the NMR data are the following: s, singlet; d, doublet; dd, doublet of doublets; ddd, doublet of doublet of doublets; td, triplet of doublets; and m, multiplet. Coupling constants are reported as J in Hz. The number of protons (n) for a given resonance is indicated by nH. NMR spectra, mass spectra and docking results are available in supporting material .
5
Isolation and Characterization of Amphidinium Species
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General Experimental Procedures Optical rotation and IR data were measured on a JASCO DIP-370 polarimeter and a JASCO FT/IR-5300 spectrophotometer, respectively. NMR data were recorded using 2.5 mm microcells (Shigemi Co., Ltd., Japan). NMR spectra were measured on a Bruker AMX-500 spectrometer or a Varian-NMR500 spectrometer equipped with a triple resonance PFG Cold Probe or CH "Xsens" PFG Cold Probe. Chemical shifts were reported in ppm with reference to the residual proton and carbon signals of C 6 D 6 (δ H 7.20 and δ C 128.0, respectively) and CDCl 3 (δ H 7.26 and δ C 77.0, respectively). ESI-MS spectra were recorded on a JEOL JMS-T100LC spectrometer.
Materials A dinoflagellate Amphidinium species (strain KCA09053) was monoclonally separated from benthic sea sands collected off Iriomote Island, Japan in May 2009. 6) (link) The voucher specimen was deposited at the Center for Advanced Marine Core Research, Kochi University.
Materials A dinoflagellate Amphidinium species (strain KCA09053) was monoclonally separated from benthic sea sands collected off Iriomote Island, Japan in May 2009. 6) (link) The voucher specimen was deposited at the Center for Advanced Marine Core Research, Kochi University.
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