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Pcmv6 entry c terminal myc ddk tags

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PCMV6-Entry (C-terminal Myc-DDK tags) is a mammalian expression vector designed for cloning and expression of proteins with C-terminal Myc and DDK tags. The vector contains the cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cell lines.

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Lab products found in correlation

Pcmv6 ac gfp, by OriGene (2 mentions) Pcmv6 an gfp, by OriGene (2 mentions) Hek293f cell line, by Thermo Fisher Scientific (2 mentions) Pcmv6 an myc ddk, by OriGene (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PCMV6-Entry (97 citations) Myc-DDK tag (21 citations) PCMV6-ddk-myc (21 citations) DDK tag (4 citations) C-terminal DDK tag (3 citations) Myc tag (3 citations) C-terminal MYC/DDK tag (2 citations) DDK-Myc tags (2 citations) PCMV6-c-myc (2 citations) C-Myc (2 citations)

2 protocols using pcmv6 entry c terminal myc ddk tags

1

Overexpression of PRR14L in HEK293F

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The HEK293F cell line (Thermo Fisher Scientific, Waltham, MA, USA) was grown in DMEM plus 10% fetal calf serum and transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Full length PRR14L (Genbank accession: NM_173566) was synthesized (Genescript, New Jersey, USA) and transferred to pCMV6-Entry (C-terminal Myc-DDK tags; Origene Technologies Inc., Maryland, USA). Wildtype and mutant PRR14L were then transferred to pCMV6-AN-Myc-DDK, pCMV6-AC-GFP and pCMV6-AN-GFP (Origene Technologies Inc, Maryland, USA).
Chase A., Pellagatti A., Singh S., Score J., Tapper W.J., Lin F., Hoade Y., Bryant C., Trim N., Yip B.H., Zoi K., Rasi C., Forsberg L.A., Dumanski J.P., Boultwood J, & Cross N.C. (2018). PRR14L mutations are associated with chromosome 22 acquired uniparental disomy, age related clonal hematopoiesis and myeloid neoplasia. Leukemia.
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2

Overexpression of PRR14L in HEK293F

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The HEK293F cell line (Thermo Fisher Scientific, Waltham, MA, USA) was grown in DMEM plus 10% fetal calf serum and transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Full length PRR14L (Genbank accession: NM_173566) was synthesized (Genescript, New Jersey, USA) and transferred to pCMV6-Entry (C-terminal Myc-DDK tags; Origene Technologies Inc., Maryland, USA). Wildtype and mutant PRR14L were then transferred to pCMV6-AN-Myc-DDK, pCMV6-AC-GFP and pCMV6-AN-GFP (Origene Technologies Inc, Maryland, USA).
Chase A., Pellagatti A., Singh S., Score J., Tapper W.J., Lin F., Hoade Y., Bryant C., Trim N., Yip B.H., Zoi K., Rasi C., Forsberg L.A., Dumanski J.P., Boultwood J, & Cross N.C. (2018). PRR14L mutations are associated with chromosome 22 acquired uniparental disomy, age related clonal hematopoiesis and myeloid neoplasia. Leukemia.
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