Ab92547

Manufactured by R&D Systems

Ab92547 is a laboratory equipment product offered by R&D Systems. It is designed for laboratory use and serves a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Axio vert a1, by Zeiss (1 mentions) Ab7817, by Santa Cruz Biotechnology (1 mentions) Gata4, by Santa Cruz Biotechnology (1 mentions) Alexa fluorogenic secondary antibodies, by Thermo Fisher Scientific (1 mentions) Af1062, by Abcam (1 mentions) A21422, by Thermo Fisher Scientific (1 mentions) Mab1757, by R&D Systems (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Sylgard 184 elastomer, by Dow (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Ingenuity pathway analysis (ipa), by Merck Group (1 mentions) Alexafluor 647, by R&D Systems (1 mentions) Ip l 780, by Nanoscribe (1 mentions)

3 protocols using ab92547

Immunofluorescent Characterization of Cardiac Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed in 4% paraformaldehyde for 15 min, permeabilized with PBS buffer containing 0.2% Triton-X for 30 min and blocked with 3% normal donkey serum for 2 h at room temperature. Then, cells were incubated with primary antibodies against Vimentin (Abcam, ab92547, 1:400), Pdgfrα (R&D, AF1062, 1:50), αSMA (Abcam, ab7817, 1:400), Gata4 (Santa Cruz, sc-25310, 1:100), Mef2c (CST, 5030s, 1:400), Tbx5 (Invitrogen, 42-6500, 1:200), cTnT (Thermo Fisher Scientific, MA5-12960, 1:200), cTnI (Abcam, ab56357, 1:300), and α-Actinin (Sigma, A7311, 1:500) at 4°C overnight. The next morning, after washing with PBS, Alexa fluorogenic secondary antibodies (Invitrogen, 1:1,000) were incubated for 1 h at room temperature to detect a signal. DAPI (1: 10,000) was finally used to determine the nuclear localization. Images were captured by inverted fluorescence microscopes from Zeiss (AXIO Vert.A1) and Nikon (Ti-E).

Zhao H., Zhang Y., Xu X., Sun Q., Yang C., Wang H., Yang J., Yang Y., Yang X., Liu Y, & Zhao Y. (2021). Sall4 and Myocd Empower Direct Cardiac Reprogramming From Adult Cardiac Fibroblasts After Injury. Frontiers in Cell and Developmental Biology, 9, 608367.

+ Open protocol

+ Expand

Quantifying Kidney Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence studies were conducted to determine the level of NGAL and Vimentin in the kidney tissue. Slides of kidney tissue were fixed, permeated, and blocked with an antibody against Vimentin (Abcam, ab92547) and NGAL (MAB1757, R&D Systems) overnight. The sections were stained with a secondary antibody: anti-mouse for the Vimentin, anti-goat for the NGAL, and Alexa 555, a fluorochrome (A21422, Molecular Probes), for 1 hour. Confocal microscopy was performed as described previously (Halade et al., 2018a (link)).

Krishnan V., Booker D., Cunningham G., Jadapalli J.K., Kain V., Pullen A.B, & Halade G.V. (2018). Pretreatment of Carprofen Impaired Initiation of Inflammatory- and Overlapping Resolution Response and Promoted Cardiorenal Syndrome in Heart Failure. Life sciences, 218, 224-232.

+ Open protocol

+ Expand

Photocrosslinkable Resin for 3D Microstructures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Photocrosslinkable resin IP-L 780 (Nanoscribe GmbH) was used for producing master 3D microstructures by 2PP. The structures were developed using propylene glycol monomethyl ether acetate (PGMEA, RER600, Arch Chemicals) and rinsed using isopropyl alcohol (IPA, Sigma-Aldrich). Polydimethylsiloxane (PDMS, Sylgard 184 elastomer, Dow Corning) and fluoroctatrichlorosilane (FOTS, Sigma-Aldrich) were used for microlithography procedure to finally emboss PLA films (Corbion Purac®, Corbion), providing them with microstructures. Components for cell culture such as Minimum Essential Medium α (α-MEM), fetal bovine serum (FBS), penicillin-streptomycin (Pen/Strep), L-glutamine (L-glu) and trypsin in ethylenediaminetetraacetic acid (trypsin-EDTA) were from Gibco, Life Technologies. Bovine serum albumin (BSA) was from Sigma-Aldrich. For fluorescence microscopy, the following probes were used: fluorescein isothiocyanate (FITC) conjugated antibody for vinculin (F7053, Sigma-Aldrich), antivimentin (ab92547) primary antibody and anti-rabbit Alexa Fluor® 647 as secondary antibody (from R&D Systems), CF™594 phalloidin (Biotium) for cytoskeleton, 4′,6-Diamidino-2-phenylindole dihydrochloride for nuclei (DAPI, Sigma-Aldrich).

Barata D., Dias P., Wieringa P., van Blitterswijk C, & Habibovic P. (2017). Cell-instructive high-resolution micropatterned polylactic acid surfaces. Biofabrication, 9(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!