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Phenogel columns

Manufactured by Shimadzu
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Sourced in Japan

Phenogel columns are size-exclusion chromatography (SEC) columns designed for the analysis of polymers and macromolecules. They are used to separate molecules based on their size and molecular weight, providing information about the molecular weight distribution of a sample.

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Lab products found in correlation

500 mhz nmr spectrometer, by JEOL (1 mentions) Spectrum 2000 ftir spectrophotometer, by PerkinElmer (1 mentions)

2 protocols using phenogel columns

1

Polymer Characterization by GPC, NMR, and FT-IR

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Gel permeation chromatography (GPC) was conducted on a Waters GPC System (Shimadzu, Japan) equipped with two Phenogel columns (103 and 105 Å) (size: 300 × 7.80 mm) in series and a refractive index detector. HPLC grade THF was used as an eluent at a flow rate of 1 mL/min at 40 °C. Poly(methyl methacrylate) standards were used to generate a calibration curve.
1H and 13C nuclear magnetic resonance (NMR) spectra were obtained using a 500-MHz NMR spectrometer (JEOL, Japan) at room temperature. The chemical shift was referenced to the solvent peak of CDCl3 at 7.3 ppm.
Fourier transform infrared (FT-IR) spectra were collected using a Spectrum 2000 FT-IR spectrophotometer (PerkinElmer, USA) at room temperature. The copolymers were mixed with KBr and tableted, undergoing 32 scans in the wavenumber range of 4000–400 cm−1 at a resolution of 4 cm−1.
Han Y., Jiang L., Shi H., Xu C., Liu M., Li Q., Zheng L., Chi H., Wang M., Liu Z., You M., Loh X.J., Wu Y.L., Li Z, & Li C. (2021). Effectiveness of an ocular adhesive polyhedral oligomeric silsesquioxane hybrid thermo-responsive FK506 hydrogel in a murine model of dry eye. Bioactive Materials, 9, 77-91.
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2

SEC Analyses of Polymer Samples

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SEC analyses were performed using a UFLC Shimadzu Prominence SEC system
equipped with Phenogel™ columns (5 μm, 104 Å and
105 Å). Dimethylacetamide (DMAc) containing
butylhydroxytoluene (BHT, 0.05 % w/w) and LiBr (0.03 % w/w) was used as the
eluent at a flow rate of 1 ml min−1 at 50 °C. SEC
samples were prepared by dissolution in DMAc and were passed through a 0.22
μm syringe filter (nylon) before injection. Apparent molecular weights
were derived from calibration with near monodisperse poly(methyl methacrylate)
(PMMA) samples.
Ramamurthi P., Zhao Z., Burke E., Steinmetz N.F, & Müllner M. (2022). Tuning the Hydrophilic – Hydrophobic Balance of Molecular Polymer Bottlebrushes Enhances their Tumor Homing Properties. Advanced healthcare materials, 11(12), e2200163.
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