Anti dig ab clone 9h27l19

Cetuximab or anti‐human IgG1‐Kappa isotype control (Abinvivo; 1 mg) was labeled with digoxigenin (DIG) by incubating 1 mg Ab and 50 μg DIG‐NHS‐ester (Thermo Fisher Scientific) using a similar method to IR700 conjugation. The resulting DIG‐labeled Abs were abbreviated as cetuximab‐DIG and isotype‐DIG, respectively. Tumor‐bearing mice were injected with cetuximab‐DIG or isotype‐DIG (100 μg) into the lateral tail vein. Tumors were harvested 24 h after injecting DIG‐labeled Abs. The distribution of DIG‐labeled Abs was analyzed in FFPE sections by multiplex immunohistochemistry using anti‐DIG Ab (clone 9H27L19; Thermo Fisher Scientific).

Anti‐ICAM‐1 Ab (clone R6‐5‐D6; Bio X Cell) or its mouse IgG2a isotype control (clone C1.18.4; Bio X Cell) (1 mg) was labeled with DIG by incubating 1 mg Ab and 50 μg DIG‐NHS‐ester (Thermo Fisher Scientific) using a similar method to IR700 conjugation. The resulting DIG‐labeled Abs were abbreviated as ICAM‐1‐DIG and isotype‐DIG, respectively. Tumor‐bearing mice were injected with ICAM‐1‐DIG or isotype‐DIG (100 μg) into the lateral tail vein. Tumors were harvested 24 h after injecting DIG‐labeled Abs. The distribution of DIG‐labeled Abs was analyzed in FFPE sections by multiplex immunohistochemistry using anti‐DIG Ab (clone 9H27L19; Thermo Fisher Scientific).