Ab197747

Manufactured by Abcam

Ab197747 is a laboratory equipment product. It is a solid-phase immunoaffinity chromatography reagent used for protein purification.

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Lab products found in correlation

Dtm200, by R&D Systems (2 mentions) Mmp200, by R&D Systems (1 mentions) Dmp900, by R&D Systems (1 mentions) Dtm100, by R&D Systems (1 mentions)

2 protocols using ab197747

Quantification of MMP-14, TIMP-2 and MMP-2

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MMP-14, TIMP-2 and total MMP-2 tissue levels were quantified using sandwich enzyme-linked immunosorbent assay (ELISA) kits. A standard curve was run in each assay; all samples and standards were measured in duplicate and findings averaged. The TIMP-2 assay procedure was done according to the manufacturer’s instructions (DTM200 and MMP200, R&D Systems). The MMP-14 assay procedure was carried out according to the manufacturer’s instructions with the incubation time of the samples with the antibody cocktail increasing to two hours (ab197747, Abcam).

Schmitt R., Tscheuschler A., Laschinski P., Uffelmann X., Discher P., Fuchs J., Kreibich M., Peyronnet R, & Kari F.A. (2019). A potential key mechanism in ascending aortic aneurysm development: Detection of a linear relationship between MMP-14/TIMP-2 ratio and active MMP-2. PLoS ONE, 14(2), e0212859.

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Quantification of MMP and TIMP Levels

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MMP-14, TIMP-2, MMP-9, and TIMP-1 tissue levels were quantified using sandwich enzyme-linked immunosorbent assay (ELISA) kits. A standard curve was run in each assay; all samples and standards were measured in duplicate and findings were averaged. The TIMP-2, MMP-9, and TIMP-1 assay procedures were done according to the manufacturer's instructions (DTM200, DMP900, and DTM100, R&D Systems). The MMP-14 assay procedure was done according to the manufacturer's instructions with the samples in the antibody cocktail's incubation time increasing to two hours (ab197747, Abcam).

Schmitt R., Tscheuschler A., Laschinski P., Discher P., Fuchs J, & Kari F.A. (2020). Matrix Metalloproteinase-2 Isoforms Differ within the Aortic Wall of Ascending Aortic Aneurysms Associated with Bicuspid Aortic Valve. Cardiology Research and Practice, 2020, 1306425.

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