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2 protocols using cerium acetylacetonate hydrate

1

Synthesis of Metal Acetate and Nitrate Complexes

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Copper(II) acetate monohydrate (puriss. p. a., ≥99.0 %), zinc(II) acetate dihydrate (puriss. p. a., ≥99.0 %), manganese(II) acetate tetrahydrate (99+%) copper(II) nitrate hemipentahydrate, zinc(II) nitrate hexahydrate, manganese(II) nitrate tetrahydrate, cerium nitrate, magnesium(II) nitrate hexahydrate, sodium carbonate, and cerium acetylacetonate hydrate were all purchased from Sigma–Aldrich. Magnesium(II) acetate tetrahydrate (analytical) was obtained from Amresco. Ethanol (absolute 99.8 %, Certified AR) was purchased from Fischer Scientific and CO2 (CP grade) was provided by BOC. All purchased materials were used as received. Deionised water was provided in‐house.
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2

Synthesis and Functionalization of Cerium-Based Nanoparticles

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All reagents and solvents were obtained commercially and used without further purification. Cerium acetylacetonate hydrate, oleylamine, xylene, 1-ethyl-3-(3-dimethly-aminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), methylene blue, and DNase I were purchased from Sigma-Aldrich. Manganese acetylacetonate, cerium acetate hydrate, citric acid (CA), 2-morpholinoethanesulfonic acid (MES), and N, N-dimethylformamide (DMF) were purchased from Aladdin. TrypLE™ Express, Dulbecco’s Modified Eagle Medium F-12 (DMEM/F12), Neurobasal-A medium, fetal bovine serum (FBS), and B27 were purchased from Abcam. Poly-L-lysine (PLL) was purchased from Solarbio. Acetone, chloroform (CHCl3), ethanol, and ethyl ether were purchased from Sinopharm. mPEG2000-NH2 and NH2-PEG2000-FITC were purchased from Ponsure Biotechnology Company.
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