Fresh mint leaves were obtained from a local market and were washed thoroughly before use. Cobalt(ii ) nitrate hexahydrate, ferrous sulphate heptahydrate, anhydrous ferric chloride, zinc nitrate hexahydrate, lead(ii ) nitrate, ferrous sulphate and mercury(ii ) chloride were purchased from Himedia Laboratories Pvt. Ltd, India. Copper chloride, calcium chloride, magnesium chloride and sodium chloride were purchased from Sisco Research Laboratories (SRL) Pvt. Ltd, India. Silver nitrate and cadmium nitrate tetrahydrate, manganous chloride, nickel nitrate, aluminium nitrate nonahydrate, potassium nitrate, potassium carbonate and sodium hydroxide were supplied by Merck Ltd, India. Ascorbic acid, citric acid, methionine, l -glutamic acid, l -cysteine, glycine, glucose and urea were purchased from Himedia Laboratories Pvt. Ltd. Solvents such as acetone, acetyl chloride, hexane, dimethyl sulfoxide were purchased from Merck Ltd, India. Methyl iodide, tetra butyl ammonium bromide and tetra ethyl acetate were supplied by SRL Pvt. Ltd, India. All the commercially available reagent grade chemicals were used as – received.
Ferrous sulphate heptahydrate
Manufactured by HiMedia
Ferrous sulphate heptahydrate is a chemical compound with the formula FeSO4·7H2O. It is a crystalline solid that appears green in color. The compound is commonly used in various laboratory applications as a source of iron.
Automatically generated - may contain errors
Lab products found in correlation
Potassium carbonate, by Merck Group (1 mentions)
Nickel nitrate, by Merck Group (1 mentions)
Aluminium nitrate nonahydrate, by Merck Group (1 mentions)
Sodium chloride (nacl), by Sisco Research Laboratories (1 mentions)
Acetyl chloride, by Merck Group (1 mentions)
L cysteine, by HiMedia (1 mentions)
Mercury 2 chloride, by HiMedia (1 mentions)
Lead 2 nitrate, by HiMedia (1 mentions)
Cobalt 2 nitratehexahydrate, by HiMedia (1 mentions)
Hexane, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Potassium nitrate, by Merck Group (1 mentions)
Zinc nitrate hexahydrate, by HiMedia (1 mentions)
Ascorbic acid, by HiMedia (1 mentions)
Urea, by HiMedia (1 mentions)
Ferrous sulphate, by HiMedia (1 mentions)
Calcium chloride, by Sisco Research Laboratories (1 mentions)
Magnesium chloride, by Sisco Research Laboratories (1 mentions)
2 protocols using ferrous sulphate heptahydrate
1
Synthesis of Metal-Based Compounds
2
Synthesis and Characterization of SPIONs
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For the synthesis of SPIONs, the following analytical grade and extra pure chemicals were used: Ferrous sulphate heptahydrate (FeSO4.7H2O), extra pure, from HiMedia laboratories, Ferric chloride hexahydrate (FeCl3. 6H2O) from Thomas Baker PVT limited; Tetramethyl ammonium hydroxide (C4H13NO), and Formaldehyde solution (CH2O) (41%), Acetone (C3H6O) from SDFCL,SDS (Sodium dodecyl Sulphate (C12H25NaO4S) from Karnataka Fine Chem, Bangalore, and N, N Dimethylformamide (HCON(CH3) from Fisher Scientific–Qualigens, Mumbai, Curcumin (C21H20O6) from SDFCL, Chitosan (C6H11NO4)n (cell culture tested) from HiMedia, Pyridoxine hydrochloride (pure) (C8H11NO3HCL) and Curcumin from SRL (Sisco Research Laboratories) Mumbai, RPMI 1640 medium (#AL199A, HiMedia), Fetal Bovine Serum (RM10432), HiMedia), Caspase 3 (Cat No: 560901, BD Biosciences) and D-PBS (#TL1006, HiMedia). Throughout the synthesis of nanoparticles, nitrogenized double distilled water was used. For functionalization, drug loading and final polymer coating freshly prepared double distilled water was used. For giving proper agitation during incubation time a cyclo-rotator was designed to fit our purpose. For cell culture experiments, HeLa cell line was obtained from Stellixer Biotec, Bengaluru, India.
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