Fluorescein diacetate propidium iodide fda pi

Manufactured by Merck Group

Sourced in Macao, Japan

Fluorescein Diacetate/Propidium Iodide (FDA/PI) is a fluorescent dye combination used for cell viability analysis. FDA is a non-fluorescent compound that becomes fluorescent upon enzymatic hydrolysis in live cells. PI is a nucleic acid-binding dye that can only enter cells with compromised membranes. When used together, FDA labels live cells while PI labels dead cells.

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Lab products found in correlation

Dithizone, by Merck Group (1 mentions) Cobe 2991, by Terumo BCT (1 mentions) Glutamine, by Lonza (1 mentions) Pen strep, by Lonza (1 mentions) Live dead assay, by Merck Group (1 mentions)

Close spelling variants of this product

Propidium iodide (7211 citations) Fluorescein diacetate (152 citations) Fluorescein diacetate (FDA) (102 citations) PI (propidium iodide) (13 citations) FDA/PI (10 citations) Propidium (PI) (7 citations)

2 protocols using fluorescein diacetate propidium iodide fda pi

Isolation and Purification of Human Islet-Derived Extracellular Vesicles

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The use of human specimens (supernatant of cultured islets or islets discarded from clinical use) was approved by Institutional Review Board. Islet-free supernatants were obtained from freshly purified human islet preparations (n = 10) from Diabetes Research Institute (HSR-DRI), San Raffaele Scientific Institute, Milan, Italy. Islets were isolated and purified according to the automated method described by Ricordi, with local modifications [18] (link). Briefly, the pancreatic duct was cannulated with 14–20 G catheter and distended by intraductal infusion of a cold collagenase solution (Collagenase NB1 Premium Grade, Serva, Heidelberg, Germany). After digestion at 37°C in a modified Ricordi chamber, islets were purified on a Cobe 2991 (TerumoBCT, Lakewood, CO, USA) using a continuous HBSS-Ficoll (Biochrom, Berlin, Germany) gradient. Purified islet fractions were cultured in Final Wash (Mediatech Cellgro, VA, USA) plus 1% Pen/Strep, 1% Glutamine (Lonza, Basel, Switzerland), counted and their numbers expressed as number of islets normalized to a 150-µm diameter (IEQ). Final islet preparations were cultured at a density of 1000±150 IEQ/ml.
Human islet preparations were selected for purity (>70%) and viability (>70%) for EV isolation by Dithizone (Sigma-Aldrich, St. Louis, MO) and Fluorescein Diacetate/Propidium Iodide (FDA/PI; (Sigma-Aldrich, St. Louis, MO) dye analysis.

Figliolini F., Cantaluppi V., De Lena M., Beltramo S., Romagnoli R., Salizzoni M., Melzi R., Nano R., Piemonti L., Tetta C., Biancone L, & Camussi G. (2014). Isolation, Characterization and Potential Role in Beta Cell-Endothelium Cross-Talk of Extracellular Vesicles Released from Human Pancreatic Islets. PLoS ONE, 9(7), e102521.

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Comparative Cell Viability on GO-PCL Scaffolds

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LIVE/DEAD assay (Sigma-Aldrich), and CCK-8 assay were conducted to compare the cell viability and proliferation ability on the four types of scaffolds, which included simple PCL, 0.1%GO-PCL, 0.1%GO-PCL/CS-PCL and 0.1%GO-PCL/NAC-CS-PCL scaffolds. Live and dead cells were stained with fluorescein diacetate/propidium iodide (FDA/PI) (Sigma-Aldrich), respectively, and observed using a laser scanning confocal microscopy (LSCM, Nikon A1Si, Japan). Specifically, the green-stained cells were living cells, and red-stained cells were dead. Cell adhesion and morphology were observed using FESEM. Typically, 1 day after incubation, HDFs on the 0.1%GO-PCL/NAC-CS-PCL scaffolds were washed three times with PBS to remove unattached cells, and fixed with 2.5% glutaraldehyde for 2 hours. Then, they were washed again with PBS to remove residual glutaraldehyde, and dehydrated with increasing concentrations of ethanol (50% for 60 min, 70% for 30 min, 80% for 20 min, 90% for 10 min, 95% for 5 min, 100% for 5 min). Finally, all the samples were dried in air, and coated with platinum for 60 s in a vacuum and observed using FESEM.

Liu J., Hou J., Liu S., Li J., Zhou M., Sun J, & Wang R. (2021). Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects. International Journal of Nanomedicine, 16, 3803-3818.

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