Sv30010

MCF-7 cells (American Type Culture Collection (ATCC), Manassas, VA) were cultured in phenol-red free Eagle's Minimum Essential Medium (MEM, Corning cellgro, 17305-CV) supplemented with 0.1 mM nonessential amino acids, 1 mM sodium pyruvate, 1% penicillin–streptomycin (Hyclone, SV30010), 1.5 g/L sodium bicarbonate (Corning cellgro, 25-035-CL), 1% l-glutamine (25-005-CI), and 10% fetal bovine serum (Corning cellgro, 35-010-CV). Human Embryonic Kidney 293 (HEK293) cells (ATCC) were cultured in phenol-red free Dulbecco's Modified Essential Medium (DMEM, Corning cellgro, 17-205-CV) supplemented with 1% l-glutamine, 1% penicillin–streptomycin, and 10% fetal bovine serum.

Murine colon cancer MC38 cells were obtained from Dr. Richard J. Barth (Geisel School of Medicine). Cell lines are tested routinely for mycoplasma by PCR at the time of experiments and retested periodically. After thawing, cells were passaged 2-3 times before use. MC38 cells were cultured in complete DMEM with a high glucose concentration (GE Hyclone Laboratories, SH30022.01) supplemented with 10% heat-inactivated FBS (GE Hyclone Laboratories, SH30910.03), 100U/ml penicillin, 100μg/ml streptomycin (GE Hyclone Laboratories, SV30010), 1mM sodium pyruvate (Corning Cellgro, 25-000-Cl), 10mM HEPES (Corning Cellgro, 25-060-Cl), 0.1mM MEM non-essential amino acids (Corning Cellgro, 25-025-Cl) and 50μM 2-mercaptoethanol (Sigma, M6250-100ML).