For apoptosis measurements, the percentages of apoptotic cells were determined by flow cytometry using the Annexin V-FITC/PI cell apoptosis detection kit (Promega) according to the manufacturer’s instructions. The Q2 and Q3 were identified as the apoptotic quadrant.
Annexin 5 fitc pi cell apoptosis detection kit
Manufactured by Promega
The Annexin V-FITC/PI cell apoptosis detection kit is a tool used to detect and quantify apoptosis in cell populations. It utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a DNA-binding dye, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.
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2 protocols using annexin 5 fitc pi cell apoptosis detection kit
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Annexin V-FITC/PI Apoptosis Assay
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Annexin V-FITC/PI Apoptosis Assay
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For apoptosis measurements, the percentages of apoptotic cells were determined by flow cytometry using the Annexin V-FITC/PI cell apoptosis detection kit (Promega) according to the manufacturer's instructions.
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