Total RNA was extracted from snap frozen dissected prefrontal cortex tissue by using the RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions. cDNA was generated by MMLV reverse transcription (Promega Germany). Quantitative Real Time PCR assays were carried out by using QuantStudio 5 (Thermo Fisher Scientific, Germany) and GoTaqaPCR Master Mix with SYBR green fluorescence (Promega, Germany). PCR primer sequences were retrieved from the Primer Bank database (Spandidos et al., 2010 (link)). Expression of genes was normalized to the expression of the housekeeping genes (Hprt1, Rpl13a, Eef2) and to the STING WT by using the ΔΔCt method. Sequences of primers are listed in the Key Resources Table.
Gotaqapcr master mix with sybr green fluorescence
Manufactured by Promega
Sourced in Germany
GoTaq qPCR Master Mix with SYBR green fluorescence is a ready-to-use solution containing all the necessary components for real-time quantitative PCR (qPCR) amplification, including a DNA polymerase, dNTPs, and SYBR green dye for fluorescent detection.
Automatically generated - may contain errors
2 protocols using gotaqapcr master mix with sybr green fluorescence
1
Quantitative PCR Analysis of Gene Expression
2
Quantitative Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from snap frozen dissected prefrontal cortex tissue by using the RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. cDNA was generated by MMLV reverse transcription (Promega Germany). Quantitative Real Time PCR assays were carried out by using QuantStudio 5 (Thermo Fisher Scientific, Germany) and GoTaq®aPCR Master Mix with SYBR green fluorescence (Promega, Germany). PCR primer sequences were retrieved from the Primer Bank database (Spandidos et al., 2009) .
Expression of genes was normalized to the expression of the housekeeping genes (Hprt1, Rpl13a, Eef2) and to the STING WT by using the ΔΔCt method. Sequences of primers are listed in supplemental Table S2.
Expression of genes was normalized to the expression of the housekeeping genes (Hprt1, Rpl13a, Eef2) and to the STING WT by using the ΔΔCt method. Sequences of primers are listed in supplemental Table S2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!