Cyquant proliferation analysis kit

Manufactured by Thermo Fisher Scientific

The CyQuant® Proliferation Analysis Kit is a laboratory product designed to quantify and analyze cell proliferation. The kit utilizes a fluorescent dye that binds to cellular nucleic acids, allowing for the measurement of cell numbers in a sample. The core function of this kit is to provide a reliable and quantitative method for assessing cell growth and proliferation in various experimental settings.

Automatically generated - may contain errors

Lab products found in correlation

Coulter particle counter, by Beckman Coulter (1 mentions) Oleic acid albumin, by Merck Group (1 mentions) 12 well plates, by Genesee Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

4 protocols using cyquant proliferation analysis kit

High-throughput shRNA screen in RCC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The top 195 genes overexpressed in RCC samples along with control genes were used to design a high-throughput shRNA screen. MISSION ® shRNA particles were prepared and plated individually by Sigma-Aldrich in Corning black, clear-bottom 96-well plates. ACHN, Caki1, Caki2, and KIJ265T cells were plated at 5000 cells per well (for a 200 MOI) in phenol red-free DMEM with 10% FBS, 1% PSA and 1X glutamax. Triplicates were on 3 separate plates. After 72 hours, plates were washed with PBS, and stored at -80ΰC prior to analysis using CyQuant ® Proliferation Analysis Kit (Invitrogen) as per manufacturers' protocol for relative fluorescence units.

von Roemeling C.A., Marlow L.A., Radisky D.C., Rohl A., Larsen H.E., Wei J., Sasinowska H., Zhu H., Drake R., Sasinowski M., Tun H.W, & Copland J.A. (2014). Functional genomics identifies novel genes essential for clear cell renal cell carcinoma tumor cell proliferation and migration. Oncotarget, 5(14), 5320-5334.

+ Open protocol

+ Expand

Cell Proliferation Assay with Oleic Acid

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded at 5,000 cells/well in clear-bottom 96-well plates in triplicate. Drug treatment was applied at 1:1000 in reduced serum conditions (3%). After 72 hours, cells were washed with PBS, and stored at -80^oC prior to analysis using CyQuant® Proliferation Analysis Kit (Invitrogen) er manufacturers’ protocol for relative fluorescence units. Alternatively, cells were plated 2x10⁵/well in 12-well plates (Genesee Scientific) in triplicate prior to drug treatment. After 120-hour treatment, cell number was established using a Coulter Particle Counter (Beckman). Oleic acid-albumin (Sigma Aldrich) was added to media at 5µM, and was applied adjuvant to drug treatment. Drug stocks were prepared in DMSO (Sigma) at 1000x. IC₅₀ dosing per cell line was calculated using CalcuSyn analytical software.

von Roemeling C.A., Caulfield T.R., Marlow L., Bok I., Wen J., Miller J.L., Hughes R., Hazlehurst L., Pinkerton A.B., Radisky D.C., Tun H.W., Kim Y.S., Lane A.L, & Copland J.A. (2017). Accelerated bottom-up drug design platform enables the discovery of novel stearoyl-CoA desaturase 1 inhibitors for cancer therapy. Oncotarget, 9(1), 3-20.

+ Open protocol

+ Expand

Cell Proliferation Analysis using CyQUANT Kit

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was evaluated by using CyQUANT proliferation analysis Kit (Thermo Fisher Scientific, REF# C7026).

Zhang H., Wang D., Li M., Plecitá-Hlavatá L., D'Alessandro A., Tauber J., Riddle S., Kumar S., Flockton A., McKeon B.A., Frid M.G., Reisz J.A., Caruso P., El Kasmi K.C., Ježek P., Morrell N.W., Hu C.J, & Stenmark K.R. (2017). The Metabolic and Proliferative State of Vascular Adventitial Fibroblasts in Pulmonary Hypertension is Regulated through a MiR-124/PTBP1/PKM Axis. Circulation, 136(25), 2468-2485.

+ Open protocol

+ Expand

CyQUANT Cell Proliferation Assay Protocol

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was evaluated using CyQUANT proliferation analysis Kit (Thermo Fisher Scientific, REF# C7026) as previously described [20 (link), 23 (link)]. After various days in culture, plates were gently inverted to remove medium by blotting it onto paper towels and washed once with PBS. Cells in the plates were frozen and stored at −80 °C until samples to be assayed. Measurement was done following manufacture’s instruction. Briefly, plates were thawed at room temperature, then 250 μl of the CyQUANT GR dye/cell-lysis buffer was added to each sample well. Mixed gently, incubated at room temperature for 5 min, protected from light, then fluorescence was measured using a fluorescence plate reader with filters at 480 nm excitation and 520 nm emission maxima.

Zhang H., D’Alessandro A., Li M., Reisz J.A., Riddle S., Muralidhar A., Bull T., Zhao L., Gerasimovskaya E, & Stenmark K.R. (2023). Histone Deacetylase Inhibitors Synergize with Sildenafil to Suppress Purine Metabolism and Proliferation in Pulmonary Hypertension. Vascular pharmacology, 149, 107157.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!