High binding polystyrene 96 well elisa plates

Lauric acid was purchased from Alfa Aesar. Sodium dodecylbenzenesulfonate (SDBS) and cetyltrimethylammonium tosylate (CTAT) were purchased from TCI America and Merck, respectively. N,N′-dicyclohexylcarbodiimide (DCC) coupling reagent was purchased from TCI America. High-binding polystyrene 96-well ELISA plates were purchased from Santa Cruz Biotechnology, Inc. Biotin-ConA and biotin-SNA were purchased from Vector Laboratories. Galectin-3 with a His tag at the N-terminus was purchased from Abcam. All other chemicals were purchased from Sigma-Aldrich unless sated otherwise and used as received.

The biantennary complex-type containing sialoglycoprotein (SGP) was prepared from dried hen’s egg yolk following the reported procedure.^{53 (link)} The high mannose containing glycoprotein soybean agluttinin was prepared from soybean flour following a previously reported method.⁵⁶ Vibrio cholerae neuraminidase, Bgaa β(1,4)-galactosidase, and α(1,2)-mannosidase were overexpressed in Escherichia coli BL21-(DE3) cells following our previously reported procedure.⁵⁶ 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was purchased from Oakwood Chemical; 10 kDa MWCO spin filters were purchased from Merck. High-binding polystyrene 96-well ELISA plates were purchased from Santa Cruz Biotechnology, Inc. All other materials were purchased from Sigma-Aldrich unless stated otherwise and used as received.