Milli q gradepurification system

Invitrogen
FluoSpheres, carboxylate-modified
nanoparticles, red fluorescent (580/605), and 2% solids were purchased
from ThermoFisher scientific at various sizes (20,100,200,500,1000
nm). In addition, Invitrogen FluoSpheres, yellow-green fluorescent
(505/515), 2% solid, and 20 nm carboxylate-modified nanoparticles
were also purchased for separation experiments from the same supplier.
Lithium chloride salt (LiCl, 99%) used for diffusiophoresis experiments
was purchased from Acros Organics. Aqueous liposome solutions were
prepared with buffer salt, HEPES and chelating agent EDTA purchased
from Sigma-Aldrich. Lipophilic dye, 3,3′-dioctadecyloxacarbocyanine
perchlorate (DiO_c18) (used
for staining liposomes) and chloroform (99%) (used for preparing lipid
films) were purchased from Sigma-Aldrich. RTV 615 polydimethylsiloxane
used for the fabrication of microfluidic devices was purchased from
Techsil, UK. The phospholipids 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC), and 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) were purchased from Avanti Polar Lipids. Deionized
water (18.2 MΩ cm) produced from an ultrapure milli-Q grade
purification system (Millipore, USA) was used to prepare all aqueous
solutions.

RTV 615 PDMS was purchased from Techsil,
UK, and photoreactive Norland Optical Adhesive (NOA) 81 was purchased
from Norland Products Inc., USA. The suspensions of colloidal particles
were prepared with carboxylate-modified 200 nm polystyrene fluorescent
colloidal spheres (Fluoresbrite YG, Polysciences, USA) and lithium
chloride salt (LiCl, 99%) purchased from Acros Organics. Aqueous solutions
were prepared with DI water (resistivity 18.2 MΩ cm) produced
from an ultrapure Milli-Q grade purification system (Millipore, USA).
The chlorinated solvent (anhydrous dichloromethane, purity ≥
99.8%), used for recovery of the microgrooved substrate, was purchased
from Sigma-Aldrich, USA.