sgID-BC amplicon libraries were prepared from genomic DNAs by single-step PCR amplification with NEBNext Ultra II Q5 Master Mix (M0544L) using dual indexing primer pairs. PCR products were purified first using MinElute PCR Purification Kit (Qiagen 28006), then with Agencourt AMPure XP beads (Beckman Coulter A63881) and assessed for quality with Agilent High Sensitivity DNA kit on the Agilent 2100 Bioanalyzer prior to sequencing on the Illumina MiSeq Nano platform (Admera Health Biopharma Services).
Miseq nano platform
The Illumina MiSeq Nano platform is a benchtop sequencing system designed for small-scale sequencing projects. It is capable of generating high-quality sequencing data using Illumina's proprietary sequencing-by-synthesis technology. The MiSeq Nano platform is intended for researchers and laboratories requiring a compact and cost-effective sequencing solution.
Lab products found in correlation
2 protocols using miseq nano platform
Laser Capture Microdissection and DNA Extraction from FFPE Tissue
Laser Capture Microdissection and DNA Extraction from FFPE Tissue
sgID-BC amplicon libraries were prepared from genomic DNAs by single-step PCR amplification with NEBNext Ultra II Q5 Master Mix (M0544L) using dual indexing primer pairs. PCR products were purified first using MinElute PCR Purification Kit (Qiagen 28006), then with Agencourt AMPure XP beads (Beckman Coulter A63881) and assessed for quality with Agilent High Sensitivity DNA kit on the Agilent 2100 Bioanalyzer prior to sequencing on the Illumina MiSeq Nano platform (Admera Health Biopharma Services).
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