The concentrations of MIF, HPA-1, CD138, MMP-9, IL-6 and IL-8 in the serum or cell culture medium were measured using human MIF, HPA-1, CD138, MMP-9, IL-6 and IL-8 ELISA kits (R&D Systems) following the manufacturer’s instructions. The concentrations of MIF, HPA-1, MMP-9 and CD138 in the serum or peritoneal lavage fluid of mice were measured using mouse MIF, HPA-1, MMP-9 and CD138 ELISA kits (BlueGene Biotech, Shanghai, China). NS1 ELISA was carried out using paired anti-NS1 antibodies prepared in our laboratory and was quantified by the addition of 100 μl of 3,3',5,5'-tetramethylbenzidine (TMB) substrate (R&D Systems).
Human mif
Manufactured by R&D Systems
Sourced in United States
Human MIF, a recombinant protein produced in E. coli, is a cytokine that functions as a regulator of innate immune and inflammatory responses. It is involved in the activation of the immune system, the recruitment of inflammatory cells, and the modulation of cytokine production.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human mif
1
Quantifying Protein Biomarkers in Biological Samples
2
Biomarker Profiling in Cardiovascular Disease
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Venous blood was collected from all the patients into anticoagulant heparin tubes in the following morning of the admission day. While for the DCS subgroup above, the venous blood was extracted every 2 hours within the first 24 hours (day 1) soon after admission, and then every other day until day 9 (in other words the blood samples were collected in day 1, day 3, day 5 day 7, and day 9 for DCS subgroup). Plasma was separated and stored at -80°C until analysis. MIF levels were determined by ELISA with commercially available kits (Human MIF, Quantikine, R&D Systems, Minneapolis, USA). The levels of high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), C-reactive protein (CRP), cTnT, CK-MB, CK, B-type natriuretic peptide (BNP) and white blood cells (WBC) were assayed by our hospital's centre clinical laboratory.
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