Reagent for cell culture

The LLM was purchased from Cayman Chemical Company (Ann Arbor, MI). The reagent for cell culture were purchased from Life Technologies-Thermo Fisher Scientific (Waltham, MA). Antibodies against cMyc, hexokinase II (HKII), pyruvate kinase M2 (PKM2), and lactate dehydrogenase A (LDH-A) were purchased from Cell Signaling Technology (Danvers, MA). The antibodies against glucose transporter 1 (Glut1) and Cyclin D1 were purchased from Abcam (Cambridge, MA) and Santa Cruz Biotechnology (Dallas, TX), respectively. An antibody against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purchased from GeneTex (Irvine, CA). The anti-β-Actin antibody was purchased from Sigma-Aldrich (St. Louis, MO). Alexa Fluor 488-conjugated goat anti-rabbit antibody was from Life Technologies. A kit for colorimetric measurement of lactate was purchased from BioVision (Milpitas, CA).

LLM (purity ≥ 98%) was purchased from Cayman Chemical Company (Ann Arbor, MI). Dehydroabietyl isothiocyanate (LLM-ITC) was purchased from American Custom Chemicals Corp (San Diego, CA), whereas enzalutamide was purchased from Sigma-Aldrich (St. Louis, MO). Reagent for cell culture were purchased from Life technologies-Thermo Fisher Scientific (Waltham, MA), and charcoal-dextran-stripped fetal bovine serum (cFBS) was purchased from Hyclone (GE Healthcare; Logan, UT). The synthetic androgen R1881 was purchased from Perkin- Elmer (Boston, MA). The antibody against AR was purchased from Santa Cruz Biotechnology (Dallas, TX). The anti-prostate-specific antigen (PSA; A0562) antibody was from Dako-Agilent Technologies (Carpinteria, CA). An antibody against phospho-AR (Ser210/213) was purchased from Imgenex-Novus Biologicals (Littleton, CO). FuGENE 6, Dual-Luciferase Reporter Assay kit, and pRL-CMV were purchased from Promega (Madison, WI). AR mutant plasmid pCMV-AR-Y739A was kindly provided by Dr. Elizabeth M. Wilson (University of North Carolina, Chapel Hill, USA). The rat probasin promoter plasmid p159pPr-luc was a gift from Jeffery Green (Addgene plasmid #8392).