Anti p18

Manufactured by Thermo Fisher Scientific

Anti-p18 is a laboratory equipment product used for detecting the p18 protein. It functions as a tool for researchers to identify and analyze the presence of p18 in samples.

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Lab products found in correlation

Anti bmrf1, by Merck Group (2 mentions) Hrp labeled donkey anti goat antibody, by Santa Cruz Biotechnology (1 mentions) Anti involucrin, by Merck Group (1 mentions) Anti blimp1, by Cell Signaling Technology (1 mentions) Anti δnp63, by BioLegend (1 mentions) Anti klf4, by Cell Signaling Technology (1 mentions) Hrp labeled goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions) Anti bzlf1, by Santa Cruz Biotechnology (1 mentions) Anti c myc, by Abcam (1 mentions) Ab90543, by Abcam (1 mentions) Ab78113, by Merck Group (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti nfatc1, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions)

2 protocols using anti p18

Detailed Antibody Use for Immunoblot Analysis

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The following antibodies were used for immunoblot analyses in this study: anti-R rabbit polyclonal antibody directed against the R peptide (peptide sequence EDPDEETSSQAVKALREMAD 1: 2500), anti-p63ɑ (Cell Signaling Technologies catalog # 13109 1:1000), anti-ΔNp63 (Biolegend, catalog #619001), anti-KLF4 (Cell Signaling Technologies, catalog #4038 1: 1000), anti-BZLF1 (Santa Cruz, catalog # sc-53904, 1:500), anti-BMRF1 (Millipore, catalog # MAB8186, 1: 2500), anti-p18 (Thermo-Fisher Scientific, # PA1-73003, 1:2000), anti-BLIMP1 (Cell Signaling Technologies catalog # 9115S 1:1000), anti-involucrin (Sigma, catalog #19018, 1:3000), anti-c-myc (Abcam catalog# ab32072, 1:10000), p38 MAPK14 antibody (Cell Signaling Technologies catalog#9218 1:1000) and phospho-p38 MAPK (Cell Signaling Technologies catalog #9211 1:1000), anti-EBV SM polyclonal rabbit antibody (1:750), a generous gift from Sankar Swaminathan of the University of Utah, anti-Tristetraprolin (Cell Signaling Technologies catalog# #71632 1:1000). The secondary antibodies used were Horseradish peroxide (HRP)- labeled goat anti-mouse antibody (Thermo Scientific# 31430, 1:5000), HRP- labeled donkey anti-goat antibody (Santa Cruz#sc-2056, 1:5000), and HRP- labeled goat anti-rabbit antibody (Fisher Scientific 1:10000).

Van Sciver N., Ohashi M., Nawandar D.M., Pauly N.P., Lee D., Makielski K.R., Bristol J.A., Tsao S.W., Lambert P.F., Johannsen E.C, & Kenney S.C. (2021). ΔNp63α promotes Epstein-Barr virus latency in undifferentiated epithelial cells. PLoS Pathogens, 17(11), e1010045.

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Immunoblotting Analysis of EBV Proteins

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Immunoblotting was performed as previously described [52 (link)]. The following primary antibodies were used: anti-EBNA1 (Santa Cruz #sc-81581), anti-EBNA2 (Abcam #ab90543), anti-LMP1 (Abcam #ab78113), anti-β-actin (Sigma #A5441), anti-GAPDH (Cell Signaling Technology #D16H11), anti-BMRF1 (Millipore #MAB8186), anti-p18 (Thermo Scientific #PA1-73003), anti-BZLF1 (Santa Cruz #sc-53904), anti-R rabbit polyclonal antibody directed against the R peptide (peptide sequence EDPDEETSQAVKALREMAD), anti-NFATc1 (Santa Cruz sc-17834) and anti-tubulin (Sigma T5168). The secondary antibodies used were horseradish peroxidase (HRP)–goat anti-mouse (Thermo Scientific #31430) and donkey anti-goat (Santa Cruz #sc-2056). Image Studio Lite software was used to quantify levels of Z and R relative to loading control tubulin in Fig 7B.

Bristol J.A., Djavadian R., Albright E.R., Coleman C.B., Ohashi M., Hayes M., Romero-Masters J.C., Barlow E.A., Farrell P.J., Rochford R., Kalejta R.F., Johannsen E.C, & Kenney S.C. (2018). A cancer-associated Epstein-Barr virus BZLF1 promoter variant enhances lytic infection. PLoS Pathogens, 14(7), e1007179.

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