Protein concentration was measured using a Direct Detect Infrared Spectrometer (Merck). 20 µg of proteins were separated on a NuPAGE 4–12% Bis-Tris gel (Thermo Fisher Scientific). Proteins were transferred for 2 hr at 100 V, 400 mA, 100 W on an Immun-Blot Low Fluorescent PVDF Membrane (BioRad) and blocked for 1 hr in 1× LI-COR TBS Blocking Buffer (LI-COR). Primary antibodies were incubated overnight at 4°C. LI-COR secondary antibodies were incubated for 45 min at room temperature (RT) and images acquired with an Odyssey CLx scanner (LI-COR).
Immun blot low fluorescent pvdf membrane
Manufactured by Bio-Rad
The Immun-Blot Low Fluorescent PVDF Membrane is a type of polyvinylidene fluoride (PVDF) membrane used in Western blotting applications. It is designed to provide low fluorescent background, which can improve the signal-to-noise ratio during detection of proteins.
Automatically generated - may contain errors
2 protocols using immun blot low fluorescent pvdf membrane
1
Protein Quantification and Western Blotting
2
Quantitative Protein Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein concentration was measured using a Direct Detect Infrared Spectrometer (Merck). 20µg of proteins were separated on a NuPAGE 4-12% Bis-Tris gel (Thermo Fisher Scientific). Proteins were transferred for 2h at 100V, 400mA, 100W on an Immun-Blot Low Fluorescent PVDF Membrane (BioRad) and blocked for 1h in 1x Licor TBS Blocking Buffer (Licor). Primary antibodies were incubated over night at 4°C. Licor secondary antibodies were incubated for 45min at room temperature (RT) and images acquired with an Odyssey CLx scanner (Licor).
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