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2 protocols using potassium acetate

1

Antioxidant Evaluation of Natural Compounds

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2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′- azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox), Folin–Ciocalteu reagent, gallic acid, sodium carbonate, aluminum chloride, potassium acetate, quercetin, acetic acid, hexane, metaphosphoric acid, and 2,6-dichlorophenol indophenol sodium salt (all chemicals of AR grade) were procured from Sisco Research Laboratories Pvt. Ltd. (SRL), Mumbai, India. Throughout the experiment, deionized water that had been filtered through a 0.22 μm filter was used.
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2

Glycosaminoglycan Enzymatic Modification

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D-Glucuronic acid, chondroitin sulphate B, chondroitinase ABC, chondroitinase AC, Streptomyces hyaluronidase, β-D-galactosidase, sodium dodecyl sulphate, coomassie blue R-250, N,N-methylene bisacrylamide, and ß-mercaptoethanol were purchased from Sigma Chemical Company, St. Louis, USA. Pepsin, proteinase K, and potassium acetate were procured from Sisco Research Laboratory, Mumbai, India. All other reagents were of high analytical grade.
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