The culture medium used for the endothelial cells has been previously described (Sano et al., 2010 ). Polyclonal anti‐claudin‐5 and anti‐occludin antibodies were purchased from Zymed (San Francisco, CA, USA). Polyclonal antibeta tubulin was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Fingolimod and Fingolimod‐phosphate were provided by Mitsubishi Tanabe Pharma (Osaka, Japan). S1P was purchased from Avanti Polar Lipids (Avanti Polar Lipids Inc., AL, USA).
Polyclonal anti claudin 5
Manufactured by Zymo Research
Sourced in United States
The Polyclonal anti-claudin-5 is a laboratory reagent used for the detection and analysis of the claudin-5 protein. Claudin-5 is a tight junction protein that plays a crucial role in the regulation of the blood-brain barrier. This polyclonal antibody is designed to specifically bind to and recognize the claudin-5 protein, allowing researchers to study its expression and distribution in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti occludin antibodies, by Zymo Research (3 mentions)
Fingolimod, by Mitsubishi (2 mentions)
Fingolimod phosphate, by Mitsubishi (2 mentions)
Sphingosine 1 phosphate (s1p), by Avanti Polar Lipids (1 mentions)
Anti tnf α, by R&D Systems (1 mentions)
Anti il 17, by R&D Systems (1 mentions)
Anti ifn γ, by R&D Systems (1 mentions)
Anti vegf, by R&D Systems (1 mentions)
Polyclonal anti actin, by Santa Cruz Biotechnology (1 mentions)
Anti β tubulin, by Santa Cruz Biotechnology (1 mentions)
3 protocols using polyclonal anti claudin 5
1
Endothelial Cell Culture and Protein Analysis
2
Immunological Biomarkers in Cell Culture
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The culture medium for Cells was previously described [19] . Polyclonal anti-claudin-5 and anti-occludin antibodies were purchased from Zymed (San Francisco, CA, U.S.A). The polyclonal anti-actin, antibodies were obtained from Santa Cruz (Santa Cruz, CA, U.S.A). The polyclonal anti-TNF-α, anti-IFN-γ, anti-IL-17, anti-VEGF and anti- vascular cell adhesion molecule-1 (VCAM-1) antibodies were purchased from R&D systems (Minneapolis, MN, U.S.A).
3
Evaluating Endothelial Cell Barrier Proteins
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The culture medium for the cells has been previously described [11 (link)]. Polyclonal anti-claudin-5 and anti-occludin antibodies were purchased from Zymed (San Francisco, CA, U.S.A). Polyclonal anti-actin, anti-beta tubulin, anti-p65 subunit of NFκB antibodies, monoclonal anti-intercellular adhesion molecule-1 (ICAM-1) and anti-melanoma cell-adhesion molecule (MelCAM) antibodies were obtained from Santa Cruz (Santa Cruz, CA, U.S.A). Polyclonal anti-vascular cell adhesion molecule-1 (VCAM-1) antibodies were purchased from R&D systems (Minneapolis, MN, U.S.A). Fingolimod and Fingolimod-phosphate were provided by Mitsubishi Tanabe Pharma (Osaka, Japan).
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