Normal human bronchial epithelioid cells (16HBE cells) and NSCLC cells (A549 cells and H1299 cells) were obtained from the China Center for Type Culture Collection (Wuhan, China). H1299 cells are positive for keratin and vimentin but are negative for neurofilament triplet protein. The cells harbor a homozygous partial deletion in the p53 protein, resulting in the lack of p53 protein expression. Additionally, H1299 cells are capable of producing neuromedin B. Cells were cultured in 89% Dulbecco's modified Eagle medium (DMEM: Invitrogen) at 37°C. The siRNAs of circCPA4 (si‐circCPA4) and ASCT2 (si‐ASCT2), short hairpin RNA targeting circCPA4 (sh‐circCPA4) and negative controls, miR‐145‐5p mimic, miR‐145‐5p inhibitor and negative controls (mimic NC and inhibitor NC), and pcDNA‐ASCT2 and control (pcDNA) were purchased from GenePharma. Cell transfection was executed following the instructions of lipofectamine 2000 (Invitrogen).
16hbe cells
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
16HBE cells are a well-characterized human bronchial epithelial cell line. They are derived from normal human bronchial epithelium and exhibit characteristics of differentiated airway epithelial cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Mir 145 5p mimic, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
A549 cell, by National Collection of Authenticated Cell Cultures (1 mentions)
H1299 cells, by National Collection of Authenticated Cell Cultures (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
H1299, by National Collection of Authenticated Cell Cultures (1 mentions)
3 protocols using 16hbe cells
1
Investigating Cell-Specific Signaling in NSCLC
2
CV-A16 Infection in 16HBE Cells
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16HBE cells were purchased from the China Center for Type Culture Collection (CCTCC; Wuhan, China) and grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA), 1% each of L-glutamine, nonessential amino acids, and sodium pyruvate. Monolayers of 16HBE cells were cultured to 80% confluence in 6-well plates and either mock-incubated or incubated with the CV-A16-G20 strain (subgenotype B, GenBank: JN590244.1), isolated from an HFMD patient in Guangxi, China in 2010, at a multiplicity of infection (MOI) of 0.1 for 2 h at 37 °C in serum-free DMEM. After washing with phosphate buffered saline (PBS, pH 7.4) to remove the unadsorbed virus, the cells were maintained in DMEM with 2% FBS at 37 °C for 24 h. Each group was processed with three independent biological replicates.
3
Culturing Human Lung Cell Lines
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Human pulmonary carcinoma H441, A549, H1299 cells and bronchial epithelial 16HBE cells were purchased from China Center for Type Culture Collection, China. The cells were cultured in RPMI-1640 or DMEM medium supplemented with 10% fetal bovine serum (Gibco, USA), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco, USA) at 37 °C in humidified air containing 5% CO2.
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