V plex aβ peptide panel 1 6e10 kit

Manufactured by MSD

The V-PLEX Aβ Peptide Panel 1 (6E10) Kit is a laboratory equipment product. It is designed to quantify the levels of amyloid-beta (Aβ) peptides in biological samples using electrochemiluminescence technology.

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Lab products found in correlation

Complete protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Meso scale discovery multiplex elisa system, by MSD (1 mentions) Sector 6000, by MSD (1 mentions)

Spelling variants of this product

V-PLEX Aβ Peptide Panel 1 (6E10) (2 citations) V-PLEX Aβ peptide Panel 1 (2 citations) V-PLEX Plus Aβ Peptide Panel 1 (6E10) Kit (2 citations)

2 protocols using v plex aβ peptide panel 1 6e10 kit

Biochemical Analysis of Amyloid-Beta Levels

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Biochemical analysis of Aβ levels was performed as previously described [18 (link)]. Briefly, soluble protein was extracted from the right frontal cortex using PBS with complete protease and phosphatase inhibitor (Pierce Biotechnology). After centrifugation, the supernatant was labeled the “soluble” extract, and the pellet was homogenized in 70% formic acid. After centrifugation and neutralization with 1 M Tris-HCl, the supernatant was labeled the “insoluble” extract. Protein concentrations were determined using the BCA protein assay kit (Pierce Biotechnology). The Meso-Scale Discovery multiplex ELISA system was used to measure Aβ1–38, Aβ1–40, and Aβ1–42 levels in the soluble and insoluble extracts (V-PLEX Aβ Peptide Panel 1 (6E10) Kit; MSD).

Weekman E.M., Sudduth T.L., Price B.R., Woolums A.E., Hawthorne D., Seaks C.E, & Wilcock D.M. (2019). Time course of neuropathological events in hyperhomocysteinemic amyloid depositing mice reveals early neuroinflammatory changes that precede amyloid changes and cerebrovascular events. Journal of Neuroinflammation, 16, 284.

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Quantifying Amyloid-beta Peptides

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Aβ38/40/42 were measured by electrochemiluminescence (ECL) using a Meso Scale Discovery (MSD) V-PLEX Aβ peptide panel 1(6E10) kit, according to manufacturer’s instructions. Briefly, samples were diluted 1:2 with diluent 35 and added in duplicate to microplate wells coated with mouse monoclonal peptide specific capture antibodies for human Aβx-38/x-40/x-42. Samples were incubated with anti-Aβ antibody (6E10 clone) as the detection antibody conjugated with an electrically excitable SULFO-TAG. Measurements were made using an MSD SECTOR 6000. Concentrations were calculated from ECL signal using a four-parameter logistic curve fitting method with the MSD Workbench software package.

Arber C., Toombs J., Lovejoy C., Ryan N.S., Paterson R.W., Willumsen N., Gkanatsiou E., Portelius E., Blennow K., Heslegrave A., Schott J.M., Hardy J., Lashley T., Fox N.C., Zetterberg H, & Wray S. (2019). Familial Alzheimer’s disease patient-derived neurons reveal distinct mutation-specific effects on amyloid beta. Molecular Psychiatry, 25(11), 2919-2931.

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