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Pack diol 200 column

Manufactured by YMC
Sourced in United States, Japan

The YMC-Pack Diol-200 column is a chromatographic column designed for the separation and purification of various chemical compounds. It features a silica-based stationary phase with diol functional groups, which allows for the separation of polar and moderately polar analytes. The column is available in a variety of dimensions and can be used in both normal-phase and reversed-phase liquid chromatography applications.

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2 protocols using pack diol 200 column

1

Size-Exclusion Chromatography of Biomolecules

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The size-exclusion chromatography system was a Waters Alliance 2695 Separations Module fitted with a Waters 2996 Photodiode Array Detector (Waters Corporation, Milford, MA, USA). An YMC-Pack Diol-200 column (Product# DL20S05-5008WT, YMC America, Inc., Allentown, PA, USA) with internal dimensions of 500×8.0 mm was used at a flow rate of 0.8 ml/min. The mobile phase consisted of 0.1 M sodium phosphate and 0.15 M sodium chloride (pH 7.0), and peaks were detected at a wavelength of 214 nm. Instrument operation and data acquisition and manipulation were carried out with Waters Empower 2 Chromatography Manager (Waters Corporation) with tabulated results presented as mean +/− standard deviation of 3 separate experiments.
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2

SE-HPLC analysis of PF-06439535 HMMs

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The formation of HMMS of PF-06439535 was assessed by SE-HPLC. Isocratic elution (20 mM sodium phosphate, 400 mM sodium chloride, pH 6.0) of the samples was carried out using a YMC-Pack Diol-200 column (300 mm × 8 mm, pore size 200 Å, particle size 5 µm; YMC, Koyoto, Japan) on a Waters HPLC system (Waters Corporation, Milford, MA, USA) with ultraviolet (UV) detection and was performed at a wavelength of 280 nm.
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