The size-exclusion chromatography system was a Waters Alliance 2695 Separations Module fitted with a Waters 2996 Photodiode Array Detector (Waters Corporation, Milford, MA, USA). An YMC-Pack Diol-200 column (Product# DL20S05-5008WT, YMC America, Inc., Allentown, PA, USA) with internal dimensions of 500×8.0 mm was used at a flow rate of 0.8 ml/min. The mobile phase consisted of 0.1 M sodium phosphate and 0.15 M sodium chloride (pH 7.0), and peaks were detected at a wavelength of 214 nm. Instrument operation and data acquisition and manipulation were carried out with Waters Empower 2 Chromatography Manager (Waters Corporation) with tabulated results presented as mean +/− standard deviation of 3 separate experiments.
Pack diol 200 column
Manufactured by YMC
Sourced in United States, Japan
The YMC-Pack Diol-200 column is a chromatographic column designed for the separation and purification of various chemical compounds. It features a silica-based stationary phase with diol functional groups, which allows for the separation of polar and moderately polar analytes. The column is available in a variety of dimensions and can be used in both normal-phase and reversed-phase liquid chromatography applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pack diol 200 column
1
Size-Exclusion Chromatography of Biomolecules
2
SE-HPLC analysis of PF-06439535 HMMs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The formation of HMMS of PF-06439535 was assessed by SE-HPLC. Isocratic elution (20 mM sodium phosphate, 400 mM sodium chloride, pH 6.0) of the samples was carried out using a YMC-Pack Diol-200 column (300 mm × 8 mm, pore size 200 Å, particle size 5 µm; YMC, Koyoto, Japan) on a Waters HPLC system (Waters Corporation, Milford, MA, USA) with ultraviolet (UV) detection and was performed at a wavelength of 280 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!