1640 medium

Cell suspensions were generated by shredding and grinding the endometrial tissues in 3 ml of 1640 medium supplemented with 10% fetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd, China). After filtration and centrifugation, a mixture of 4 ml of cell suspension and 4 ml of lymphocytes separation medium (Tianjin Haoyang Biological Manufacture Co., Ltd, China) was centrifuged at 1350 g for 30 min at 20°C. After washing the samples twice, the cell suspension was incubated with anti-CD32 (0.1 μl/10⁶ cells in 100 μl buffer, BD Biosciences, USA) for 10 min at 4°C to block Fc receptors (FcRs). Samples were then incubated with biotin-conjugated DBA-lectin (1 μl/10⁶ cells in 100 μl buffer, Sigma-Aldrich, USA) for 3 min, and the suspension was incubated with FITC-avidin (0.25 μl/10⁶ cells in 100 μl buffer, BioLegend Inc., USA) for 30 min at 4°C. After the incubation period, samples were analyzed using a flow cytometer (Accuri C6, BD Biosciences, USA).

Ginkgo biloba leaves (Hebei Golden Leaf Pharmaceutical Co., ltd., Baoding, China); Acetonitrile (Fisher Chemical); Formic acid (Fisher Chemical); Methanol (Fisher Chemical); Ultrapure water (Millipore); CCl₄ (Beijing Chemical Plant, Beijing, China); HepG2 cells (CS-0009, Beijing Dingguo Changsheng Biotechnology Co., ltd., Beijing, China); Trypsin (GENVIEW); Fetal bovine Serum (Hangzhou Sijiqing Company, Hangzhou, China); MTT (American Sigma Chemical Reagents Co., ltd., USA); 1640 Medium (11011-8611, Zhejiang Tianhang Biotechnology Co., LTD); DMSO (Liaoning Quanrui Reagent Co., ltd., Jinzhou, China); TNF-α(YJ002095), IL-1β(JN301814), and IL-6(JN063159) ELISA kits (Shanghai Langton Biotechnology Co., ltd., Shanghai, China); ALT(C009-2-1), AST(C010-2-1), CAT(A007-1-1), SOD(A001-3-2), and MDA (A003-1-2) assay kits (Nanjing Jiancheng Bioengineering Research Institute, Nanjing, China).