Protein sequencing grade

All analyses were performed using a Shimadzu Prominence series HPLC system (Shimadzu Corporation, Kyoto, Japan), equipped with a LC-20AB binary pump (Serial: L20124200883), SIL-20A HT autosampler (Serial: L20345256104), CTO-20AC temperature controlled column oven (Serial: L2021525077), and CBM-20A communications bus (Serial: L20235154327). All equipment was controlled by Shimadzu LabSolutions Lite software version 5.71 SP2. For separation, an Ultra C18 column, 3 μm, 50 x 4.6 mm (RESTEK Corporation, Bellefonte, PA) was used.
Dynantin samples were analyzed at a constant solvent flow rate of 0.7 mL/min at 35 °C using a binary gradient (Table 1). Solvent A consisted of a 25% solution of acetonitrile (HPLC grade Fisher Scientific) in ddH₂O (0.2 μm filtered) and solvent B consisted of acetonitrile, with each solvent containing 0.1% trifluoroacetic acid (v/v, protein sequencing grade, Sigma Aldrich, Fairlawn NJ).
MDP samples were analyzed at a constant solvent flow rate of 1.0 mL/min at 35 °C using a binary gradient (Table 2). Solvent A consisted of ddH₂O (0.2 μm filtered) and solvent B consisted of methanol (MeOH) (HPLC grade, Fisher Scientific, Fairlawn NJ), with each solvent containing 0.1% formic acid (v/v, LC/MS grade, Fisher Scientific).

All analyses were performed using a Shimadzu Prominence series HPLC system (Shimadzu Corporation, Kyoto, Japan), equipped with an LC-20AB binary pump (Serial: L20124200883), SIL-20A HT autosampler (Serial: L20345256104), CTO-20AC temperature-controlled column oven (Serial: L2021525077), SPD-M20A photodiode array detector and CBM-20A communications bus (Serial: L20235154327). All equipment were controlled by Shimadzu Lab Solutions Lite software version 5.71 SP2. For separation, an Ultra C18 column, 3 μm, 50 × 4.6 mm (RESTEK Corporation, Bellefonte, PA) was used. Dynantin samples were analyzed at a constant solvent flow rate of 0.7 mL/min at 35 °C using a binary gradient (Table 1). Solvent A consisted of a 25% solution of acetonitrile (HPLC grade, Fisher Scientific, Fairlawn, NJ, USA) in ddH₂O (0.2 μm filtered) and solvent B consisted of acetonitrile with each solvent containing 0.1% trifluoroacetic acid (v/v, protein sequencing grade, Sigma Aldrich, Fairlawn, NJ, USA).