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Cell culture plates and flasks

Manufactured by SPL Life Sciences

Cell culture plates and flasks are laboratory equipment used to grow and maintain living cells in a controlled environment. They provide a suitable surface and nutrients for cell attachment, proliferation, and differentiation. The plates and flasks come in various sizes and well configurations to accommodate different experimental needs.

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4 protocols using cell culture plates and flasks

1

Characterization of Probiotic Lactobacillus plantarum

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Lactobacillus plantarum, a gram‐positive lactic acid bacterium, can mainly be found in fermented food and the GI tract and nowadays it is utilized in the food industry as a probiotic. L. plantarum bacteria (PTCC 1058) was purchased from the Biotechnology Department in the Iranian Research Organization for Science and Technology (IROST), Iran. Omega‐3 dietary supplement (fish oil) was purchased from Golden Seas company (Yazd, Iran). According to data provided by the supplier, the major fatty acids in this fish oil were as follows: 240 mg DHA and 360 mg EPA. Gelatin, chitosan, and gum Arabic, and other materials were purchased from Sigma‐Aldrich Ltd., Australia. MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5‐diphenyltetrazolium bromide) and dimethyl sulfoxide (DMSO) were purchased from Sigma‐Aldrich, Germany. Dulbecco's modified Eagle's medium (DMEM) and Penicillin‐Streptomycin were obtained from Biowest, Germany. Fetal bovine serum (FBS) and trypsin were purchased from Gibco, Germany. Cell culture plates and flasks were purchased from SPL, Korea, and the microtubes were purchased from Ratiolab, Germany. All primers were designed and purchased from DNA Pioneer, Korea. RNA extraction kit and complementary DNA (cDNA) synthesis kit were purchased from FAVORGEN, Taiwan, and Addbio, Korea.
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2

Differentiation of Induced Pluripotent Stem Cells

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ROCK inhibitor Y27632, dimethyl sulfoxide (DMSO), poly-L-ornithine, N6,2'-O-Dibutyryladenosine 3',5'-cyclic monophosphate (dibutyryl cAMP), dexamethasone (DEX), Dulbecco's Modified Eagle's Medium (DMEM), all-trans-retinoic acid (RA), and phosphate buffered saline (PBS) were obtained from Sigma-Aldrich Inc. (St. Louis, MO, USA). Geltrex, Neurobasal medium, Neural Induction Supplement, Advanced DMEM/F-12, StemPro Accutase Cell Dissociation Reagent, KnockOut DMEM/F-12, GlutaMAX-I Supplement, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), StemPro Neural Supplement, B-27 Serum-Free Supplement, laminin, Penicillin-Streptomycin antibiotic solution, 0.25% trypsin-EDTA solution, collagenase, and 5'-bromo-2-deoxy-uridine (BrdU) were obtained from Gibco, Thermo Fisher Scientific Inc. (Waltham, MA, USA). StemMACS iPS-Brew XF medium was obtained from Miltenyi Biotec Inc. (Bergisch Gladbach, Germany). Fetal bovine serum (FBS) was purchased from Capricorn Scientific GmbH (Ebsdorfergrund, Germany). Cell culture consumables such as cell culture plates and flasks, cell culture chamber slides, and serological pipettes were obtained from SPL Life Sciences Co. (Korea).
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3

Osteoblast Differentiation Assay Protocol

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The cell culture plates and flasks were purchased from SPL Life Sciences (Pocheon, South Korea). α-Minimum essential medium (α-MEM) was purchased from Gibco (Grand Island, NY, USA). Antibiotics (10,000 units/mL penicillin and 10,000 μg/mL streptomycin) were purchased from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Welgene (Daegu, South Korea). Compound C was purchased from Calbiochem (Darmstadt, Germany). Protease inhibitor cocktail tablets were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The primary antibodies against phospho-AMPKα (Thr172) and RUNX2 were purchased from Cell Signaling Technology (Danvers, MA, USA), and antibodies against AMPKα1/2, FOXO3a, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Invitrogen (Carlsbad, CA, USA). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified.
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4

MTT Cytotoxicity Assay Protocol

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MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and dimethyl sulphoxide were purchased from Sigma-Aldrich, USA. RPMI-1640 and penicillin-streptomycin were obtained from Biowest, Germany. Fetal bovine serum and trypsin were purchased from bioMerieux, France, and Gibco, USA. Ethidium bromide and acridine orange were purchased from Merck, Germany. Cell culture plates and flasks were purchased from SPL, Korea, and the micro tubes were purchased from Ratiolab, Germany. All primers were synthetized by Metabion, Germany. The used kits in the study include RNA extraction kit (FAVORGEN, Taiwan), cDNA synthesis kit (Addbio, Korea), and FITC Annexin V Apoptosis Detection Kit (Bioscience, USA).
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