Lactobacillus plantarum, a gram‐positive lactic acid bacterium, can mainly be found in fermented food and the GI tract and nowadays it is utilized in the food industry as a probiotic. L. plantarum bacteria (PTCC 1058) was purchased from the Biotechnology Department in the Iranian Research Organization for Science and Technology (IROST), Iran. Omega‐3 dietary supplement (fish oil) was purchased from Golden Seas company (Yazd, Iran). According to data provided by the supplier, the major fatty acids in this fish oil were as follows: 240 mg DHA and 360 mg EPA. Gelatin, chitosan, and gum Arabic, and other materials were purchased from Sigma‐Aldrich Ltd., Australia. MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5‐diphenyltetrazolium bromide) and dimethyl sulfoxide (DMSO) were purchased from Sigma‐Aldrich, Germany. Dulbecco's modified Eagle's medium (DMEM) and Penicillin‐Streptomycin were obtained from Biowest, Germany. Fetal bovine serum (FBS) and trypsin were purchased from Gibco, Germany. Cell culture plates and flasks were purchased from SPL, Korea, and the microtubes were purchased from Ratiolab, Germany. All primers were designed and purchased from DNA Pioneer, Korea. RNA extraction kit and complementary DNA (cDNA) synthesis kit were purchased from FAVORGEN, Taiwan, and Addbio, Korea.
Cell culture plates and flasks
Cell culture plates and flasks are laboratory equipment used to grow and maintain living cells in a controlled environment. They provide a suitable surface and nutrients for cell attachment, proliferation, and differentiation. The plates and flasks come in various sizes and well configurations to accommodate different experimental needs.
Lab products found in correlation
4 protocols using cell culture plates and flasks
Characterization of Probiotic Lactobacillus plantarum
Lactobacillus plantarum, a gram‐positive lactic acid bacterium, can mainly be found in fermented food and the GI tract and nowadays it is utilized in the food industry as a probiotic. L. plantarum bacteria (PTCC 1058) was purchased from the Biotechnology Department in the Iranian Research Organization for Science and Technology (IROST), Iran. Omega‐3 dietary supplement (fish oil) was purchased from Golden Seas company (Yazd, Iran). According to data provided by the supplier, the major fatty acids in this fish oil were as follows: 240 mg DHA and 360 mg EPA. Gelatin, chitosan, and gum Arabic, and other materials were purchased from Sigma‐Aldrich Ltd., Australia. MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5‐diphenyltetrazolium bromide) and dimethyl sulfoxide (DMSO) were purchased from Sigma‐Aldrich, Germany. Dulbecco's modified Eagle's medium (DMEM) and Penicillin‐Streptomycin were obtained from Biowest, Germany. Fetal bovine serum (FBS) and trypsin were purchased from Gibco, Germany. Cell culture plates and flasks were purchased from SPL, Korea, and the microtubes were purchased from Ratiolab, Germany. All primers were designed and purchased from DNA Pioneer, Korea. RNA extraction kit and complementary DNA (cDNA) synthesis kit were purchased from FAVORGEN, Taiwan, and Addbio, Korea.
Differentiation of Induced Pluripotent Stem Cells
Osteoblast Differentiation Assay Protocol
MTT Cytotoxicity Assay Protocol
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