At the age of 11 weeks, female C57BL/6 mice were actively immunized subcutaneously with 200 μg myelin oligodendrocyte glycoprotein peptide (MOG35−55) emulsified in 100 μl complete Freund's adjuvant containing 4 mg/ml Mycobacterium tuberculosis (EK-2110 kit; Hooke Laboratories, Massachusetts, USA). Directly after MOG35−55 immunization and after 24 h, mice were intraperitoneally injected with 100 ng (C57BL/6JOlaHsd donor mice adoptive T cell transfer) or 40 ng (endothelial specific knockout mice) pertussis toxin (EK-2110 kit; Hooke Laboratories) to induce a normal or a mild EAE, respectively. Both the control group and experimental group of one experiment received the same amount of PTX. Mice were weighed and clinically evaluated daily for neurological signs of the disease according to manufacturer's mouse EAE scoring guide: 0: no clinical symptoms; 0.5: distal tail paralysis; 1: tail paralysis; 2: mild paraparesis and ataxia; 2.5: moderate paraparesis; 3: complete paralysis of the hind legs; 4: paralysis to the diaphragm; 5: death by EAE.
Ek 2110 kit
Manufactured by Hooke Laboratories
Sourced in United States
The EK-2110 kit is a laboratory equipment kit designed for general purpose experimental use. It includes essential components such as a power supply, wiring, and measurement devices. The core function of the EK-2110 kit is to provide a versatile platform for conducting various scientific experiments and measurements.
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Lab products found in correlation
7 protocols using ek 2110 kit
1
Induction of Experimental Autoimmune Encephalomyelitis in Mice
2
MOG35-55 Peptide-Induced EAE Model
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Wild-type C57BL/6 females (11 weeks old) were subcutaneously immunized with 200 µg myelin oligodendrocyte glycoprotein (MOG35-55) peptide emulsified in 200µl complete Freund's adjuvant containing 5mg/ml Mycobacterium tuberculosis (EK-2110 kit; Hooke Laboratories, Massachusetts, USA). Directly after immunization and 24h later, mice received an intraperitoneal injection of 100ng pertussis toxin (EK-2110 kit; Hooke Laboratories). Mice were sacrificed on day 14 post immunization. Spinals cords were isolated and snap frozen for real-time quantitative PCR.
3
Induction of Experimental Autoimmune Encephalomyelitis
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For the induction of EAE, the mouse model of MS, animals were injected subcutaneously with an emulsion of MOG35-55 peptide in complete Freud’s adjuvant (CFA; EK-2110 kit from Hooke Laboratories), followed by intraperitoneal injection with pertussis toxin in PBS (200 ng per animal) on the day of immunization and with 24 h delay (according to manufacturer’s instructions). Control animals underwent the same treatment, but CFA without MOG35-55 peptide (CK-2110 kit from Hooke Laboratories) was used instead. Spinal cord and brains were collected at the peak of the disease when clinical score 3 (representing limp tail and complete paralysis of hind legs) has been reached. Animals that did not reach this clinical score were not analyzed in this study.
4
Chronic EAE Induction Protocol
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For the induction of chronic EAE, animals were injected subcutaneously with an emulsion of MOG35-55 in complete Freud’s adjuvant (CFA; EK-2110 kit from Hooke Laboratories) followed by the administration of pertussis toxin in PBS (0,2μg per animal), for two consecutive days (all according to manufacturer’s instructions). Spinal cords and cerebellum were collected at the peak/chronic stage of the disease with clinical score=3 representing limp tail and complete paralysis of hind legs. Animals that did not reach this clinical score were not analyzed in this study. Additional animals were injected subcutaneously with a CFA emulsion (CK-2110 kit from Hooke Laboratories) and analyzed as controls.
5
Induction of Experimental Autoimmune Encephalomyelitis
6
MOG-Induced EAE in C57BL/6 Mice
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Eleven-week-old C57BL/6JOlaHsd mice were immunized subcutaneously with 200 ng of myelin oligodendrocyte glycoprotein peptide (MOG35−55) emulsified in 100 μl complete Freund’s supplemented with 4 mg/ml of Mycobacterium tuberculosis (EK2110, Hooke Laboratories). Immediately after MOG immunization and after 24 h, mice were intraperitoneally injected with 50 ng pertussis toxin (EK2110 kit, Hooke Laboratories) to induce EAE. EAE animals were treated daily with phloretin or vehicle (50 mg/kg, intraperitoneal (ip)) after 6 days of immunization (prophylactic setup) or after disease onset (clinical score > 1, therapeutic setup). Mice were weighed and scored daily for neurological signs of the disease according to the manufacturer’s mouse EAE scoring guide: 0: no clinical symptoms, 0.5: tip of tail is limp, 1: limp tail, 1.5: limp tail and hind leg inhibition 2: limp tail and weakness of hind legs, 2.5: limp tail and dragging of hind legs, 3: limp tail and complete paralysis of the hind legs, 3.5: limp tail and complete paralysis of hind legs and mouse is unable to right itself when placed on its side, 4: paralysis to the diaphragm, 5: death by EAE.
7
Chronic EAE Induction Protocol
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