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Anti cd45 fitc clone 30 f11

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Anti-CD45-FITC (clone 30-F11) is a fluorescently-labeled monoclonal antibody that binds to the CD45 antigen. CD45 is a transmembrane glycoprotein expressed on the surface of all leukocytes. This product can be used to identify and characterize leukocyte populations in flow cytometry applications.

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Lab products found in correlation

Anti cd8 pe cy5 clone 53 6.7, by BioLegend (1 mentions) Sh800z cytometer, by Sony (1 mentions) Anti cd4 fitc clone gk1, by BioLegend (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) B220 clone ra3 6b2, by Thermo Fisher Scientific (1 mentions) Lrsii, by BD (1 mentions) Anti gr 1 pe clone rb6 8c5, by BioLegend (1 mentions) Anti sca 1 pe, by Thermo Fisher Scientific (1 mentions) Cd4 clone gk1, by Thermo Fisher Scientific (1 mentions) Anti ter119 fitc, by BioLegend (1 mentions) Anti sca1 pacific blue, by BioLegend (1 mentions) Cd3 clone 145 2c11, by Thermo Fisher Scientific (1 mentions) Ter119 clone ter119, by Thermo Fisher Scientific (1 mentions) Cd11b clone m1 70, by Thermo Fisher Scientific (1 mentions) Gr 1 clone rb6 8c5, by Thermo Fisher Scientific (1 mentions) Nk1.1 clone pk136, by Thermo Fisher Scientific (1 mentions) Anti cd11b apc clone m1 70, by BioLegend (1 mentions) Facsaria 2, by BD (1 mentions) Fcs express 6, by De Novo Software (1 mentions) Lsr fortessa cytometer, by BD (1 mentions)

5 protocols using anti cd45 fitc clone 30 f11

1

Flow Cytometry Analysis of Immune Cells

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Cell cultures and tumor samples were processed and labeled as described elsewhere.20 (link) Anti-CD4-FITC (clone GK1.5, 100406, Biolegend, Dedham, Massachusetts, USA), anti-CD3e-PE (clone 145-2C11, 12-0031-82, eBioscience, San Diego, California, USA), anti-CD69-PE-Dazzle (clone H1.2 F3, 104536, Biolegend, Dedham, Massachusetts, USA), anti-CD8-PE-Cy5 (clone 53.6–7, 100710, Biolegend, Dedham, Massachusetts, USA), anti-PD-1-PE-Cy-7 (clone 29 F.1A12, 135216, Biolegend, Dedham, Massachusetts, USA), anti-CD45-FITC (clone 30-F11, 103107, Biolegend, Dedham, Massachusetts, USA), anti-PD-L2-PE (clone MIH5, 558091, BD, Franklin Lakes, New Jersey, USA), anti-Gr-1-PE-Dazzle (RB6-8C5, 108452, Biolegend, Dedham, Massachusetts, USA), anti-CD11b-PE-Cy5 (M1/70, 101210, Biolegend, Dedham, Massachusetts, USA), anti-PD-L1-PE-Cy7 (TY-25, 107214, Biolegend, Dedham, Massachusetts, USA) were used for flow cytometric analyses under manufacturer’s indications. The analyses were performed with SH800Z cytometer (SONY, Minato, Japan).
Cervera-Carrascon V., Quixabeira D.C., Santos J.M., Havunen R., Zafar S., Hemminki O., Heiniö C., Munaro E., Siurala M., Sorsa S., Mirtti T., Järvinen P., Mildh M., Nisen H., Rannikko A., Anttila M., Kanerva A, & Hemminki A. (2020). Tumor microenvironment remodeling by an engineered oncolytic adenovirus results in improved outcome from PD-L1 inhibition. Oncoimmunology, 9(1), 1761229.
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2

Immunophenotyping of Corneal Immune Cells

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Normal and HSV-1 infected corneas at 3dpi were excised, cleaned and fixed with 4% paraformaldehyde. After washing, flat-mounted fixed corneas were washed with PBS 3 times and then blocked in 2% bovine serum albumin (BSA) followed by incubation with 1% anti-CD16/CD32 Fc receptor (FcR) mAb (BioLegend, San Diego, CA, USA) for 30 min at RT. Next, samples were stained with fluorophore-conjugated antibodies for bone marrow-derived cells, dendritic cells, macrophages and the activation cell marker by using anti-CD45 (FITC) (clone 30-F11) (BioLegend, San Diego, CA, USA), anti-CD11c (PE) (clone NL3) (BD Pharmingen, San Jose, CA), anti-F480 (AF647) (clone BM8) (BioLegend, San Diego, CA, USA) and anti-MHC class II (AF647) (clone M5/114.15.1) (BioLegend, San Diego, CA, USA) overnight at 4˚C. After three washes with PBS, corneas were mounted and imaged by using FV-1000 confocal microscope (Olympus Co, Tokyo, Japan) provided by Sistema Nacional, Ministerio de Ciencia, Tecnología e Innovación, Argentina. For quantification purposes, three images from the periphery and central flat-mounted corneas per animal were taken. Quantification of cells was performed using ImageJ software (NIH, Bethesda, MD).
Sendra V.G., Tau J., Zapata G., Lasagni Vitar R.M., Illian E., Chiaradía P, & Berra A. (2021). Polluted Air Exposure Compromises Corneal Immunity and Exacerbates Inflammation in Acute Herpes Simplex Keratitis. Frontiers in Immunology, 12, 618597.
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3

Isolation and Analysis of Mouse Hematopoietic Stem Cells

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Cells were analyzed using LRSII (BD, Pharmingen) and sorted using FACS-ARIA II (BD, Pharmingen). The following antibodies were used: anti-CD45 FITC (clone 30-F11 Biolegend), anti-CD31 FITC (clone MEC13.3 Biolegend), anti-Ter119 FITC (clone TER-119 Biolegend), anti-Sca1 Pacific Blue (clone D7 Biolegend), anti-CD51 PE (clone RMV-7 Biolegend), bio-Lineage panel antibodies [CD4 (clone GK1.5 eBioscience), CD8 (clone 53-6.7 eBioscience), CD3 (clone 145-2C11 eBioscience), Ter119 (clone TER-119 eBioscience), CD11b (clone M1/70 eBioscience), Gr1 (clone RB6-8C5 eBioscience), NK1.1 (clone PK136 eBioscience), B220 (clone RA3-6B2 eBioscience)], anti-ckit APC (clone 2B8 eBioscience), anti-Sca1 PE (clone D7 eBioscience), anti-CD34 FITC (clone RAM-34 eBioscience), anti-SLAM (CD150) PerCP cy5.5 (clone TEC15-12F12.2 Biolegend), anti-Cd11b APC (clone M1/70 Biolegend), and anti-Gr1 PE (clone RB6-8C5 Biolegend).
Guarnerio J., Mendez L.M., Asada N., Menon A.V., Fung J., Berry K., Frenette P.S., Ito K, & Pandolfi P.P. (2018). A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nature Communications, 9, 66.
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4

Characterization of Tumor-Infiltrating Immune Cells

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Tumors were harvested from mice and digested in PBS supplemented with 1.5 mg/mL of Collagenase A (Roche, Basel, Switzerland), 0.4 mg/mL DNase I (Roche), 5% FBS (Corning, Corning, NY, USA) and 10mM HEPES (Gibco, Dublin, Ireland), and incubated at 37 °C. Cells were dispersed into a single cell suspention utilizing a 70 micron cell strainer (Corning). For this study we used anti-mouse antibodies LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Invitrogen, Carlsbad, CA, USA), anti-CD45-FITC [clone 30-F11] (BioLegend, San Diego, CA, USA), anti-CD8a-PE/Cy7 [53-6.7] (BioLegend), anti-CD4- PerCP/Cyanine5.5 [GK1.5] (BioLegend), anti-XCR1-PE [ZET] (BioLegend), and anti-CD335-AF647 [29A1.4] (BioLegend), at determined titration concentrations. Samples were analyzed using a BD LSR Fortessa cytometer, and collected data were analyzed with FCS Express 6 software (De Novo Software, Pasadena, CA, USA).
Wood J., Yasmin-Karim S., Mueller R., Viswanathan A.N, & Ngwa W. (2020). Single Radiotherapy Fraction with Local Anti-CD40 Therapy Generates Effective Abscopal Responses in Mouse Models of Cervical Cancer. Cancers, 12(4), 1026.
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5

Immune Cell Profiling of Dissociated Tumors

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Dissected tumours were dissociated to obtain single-cell suspensions for antibody staining and immune cell assessment. Prior to the antibody staining, dead cells were labelled with the Fixable Viability Due eFluor™ 780 (eBiosciences, San Diego, CA, USA). The following anti-mouse antibodies were used for flow cytometry: anti-CD45-FITC (clone 30-F11, Biolegend, San Diego, CA, USA), anti-CD3-BUV395 (clone 145-2C11, BD), anti-CD4-BV421 (clone GK1.5, Biolegend), anti-CD8-PE-eFluor610 (clone 53-6.7, eBiosciences), anti-Foxp3-PE (clone FJK-16S, eBiosciences), anti-CD11b-PE-Cy7 (clone M1/70, Biolegend), anti-F4/80-BV510 (clone BM8, Biolegend), and anti-Gr1-APC (clone RB6-8C5, Biolegend).
Grzybowski M.M., Stańczak P.S., Pomper P., Błaszczyk R., Borek B., Gzik A., Nowicka J., Jędrzejczak K., Brzezińska J., Rejczak T., Güner-Chalimoniuk N.C., Kikulska A., Mlącki M., Pęczkowicz-Szyszka J., Olczak J., Gołębiowski A., Dzwonek K., Dobrzański P, & Zasłona Z. (2022). OATD-02 Validates the Benefits of Pharmacological Inhibition of Arginase 1 and 2 in Cancer. Cancers, 14(16), 3967.
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